Bovine Myosin regulatory light polypeptide 9 ELISA Kit | MYL9 elisa kit
Bovine Myosin regulatory light polypeptide 9 (MYL9) ELISA Kit
Gene Names
MYL12A; MYL9; MRCL3; MRLC2
Reactivity
Bovine
Synonyms
Myosin regulatory light polypeptide 9; Bovine Myosin regulatory light polypeptide 9 (MYL9) ELISA Kit; LC20; MGC3505; MLC2; MRLC1; MYRL2; myosin RLC|myosin regulatory light chain 1|myosin regulatory light chain 2; smooth muscle isoform|myosin regulatory light chain 9|myosin; light polypeptide 9; regu; MYL9 elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine MYL9. No significant cross-reactivity or interference between Bovine MYL9 and analogues was observed.
Sequence Length
118
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MYL9 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MYL9 in samples. An antibody specific for MYL9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,865 Da
NCBI Official Full Name
myosin regulatory light polypeptide 9
NCBI Official Symbol
MYL12A
NCBI Official Synonym Symbols
MYL9; MRCL3; MRLC2
NCBI Protein Information
myosin regulatory light polypeptide 9
UniProt Protein Name
Myosin regulatory light polypeptide 9
Protein Family
UniProt Gene Name
MYL9
UniProt Synonym Gene Names
MYRL2
Uniprot Description
MRLC2: non-sarcomeric myosin regulatory light chain 2. Binds calcium and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity. Phosphorylation of MLC-2 in the presence of calcium and calmodulin increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. Myosin is an hexamer of 2 heavy chains and 4 light chains.
Protein type: Contractile
Cellular Component: actomyosin; myosin II complex; stress fiber; Z disc
Molecular Function: actin filament binding; actin-dependent ATPase activity; ATPase activity; calcium ion binding; glutamate receptor binding
Biological Process: actomyosin structure organization; protein localization to plasma membrane; regulation of cell shape
Research Articles on MYL9
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Product Notes
The Bovine MYL9 myl9 (Catalog #AAA286010) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA286010 ELISA Kit recognizes Bovine MYL9. It is sometimes possible for the material contained within the vial of "Myosin regulatory light polypeptide 9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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