Chicken phosphatase 1 regulatory subunit 12A ELISA Kit | PPP1R12A elisa kit

Chicken Protein phosphatase 1 regulatory subunit 12A (PPP1R12A) ELISA Kit

Cat. Num. AAA285881

• Gene Names: PPP1R12A; MBSP; MYPT1
• Reactivity: Chicken
• Synonyms: phosphatase 1 regulatory subunit 12A; Chicken Protein phosphatase 1 regulatory subunit 12A (PPP1R12A) ELISA Kit; MBS; MGC133042; MYPT1; myosin phosphatase; target subunit 1; PPP1R12A elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chicken PPP1R12A. No significant cross-reactivity or interference between Chicken PPP1R12A and analogues was observed.
• Sequence Length: 1056

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PPP1R12A elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PPP1R12A in samples. An antibody specific for PPP1R12A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP1R12A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1R12A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1R12A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 45384106
• NCBI GeneID: 396020
• NCBI Accession #: NP_990454.1
• NCBI GenBank Nucleotide #: NM_205123.1
• UniProt Accession #: Q90623; Q10727; Q90624
• Molecular Weight: 106,975 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 12A
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory subunit 12A
• NCBI Official Symbol: PPP1R12A
• NCBI Official Synonym Symbols: MBSP; MYPT1
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 12A
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 12A
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R12A
• UniProt Synonym Gene Names: MBS; MYPT1; Myosin phosphatase target subunit 1

Uniprot Description

MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Cellular Component: A band; actin cytoskeleton; centrosome; cytosol; focal adhesion; kinetochore; PTW/PP1 phosphatase complex; Z disc

Molecular Function: 14-3-3 protein binding; enzyme inhibitor activity; phosphatase regulator activity; protein kinase binding

Biological Process: centrosome cycle; mitotic cell cycle; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; regulation of cell adhesion; regulation of nucleocytoplasmic transport; signal transduction