Human Trypsinogen ELISA Kit | USO1 elisa kit

Human Trypsinogen activation peptide (TAP) ELISA Kit

Cat. Num. AAA285780

• Gene Names: USO1; TAP; VDP; P115
• Reactivity: Human
• Synonyms: Trypsinogen; Human Trypsinogen activation peptide (TAP) ELISA Kit; P115; TAP; VDP; USO1 homolog; vesicle docking protein|transcytosis associated protein|vesicle docking protein p115; USO1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human USO1. No significant cross-reactivity or interference between Human USO1 and analogues was observed.
• Sequence Length: 247

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for USO1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate USO1 in samples. An antibody specific for USO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 586798211
• NCBI GeneID: 8615
• NCBI Accession #: NP_001276978.1
• NCBI GenBank Nucleotide #: NM_001290049.1
• UniProt Accession #: O60763; Q6PK63; Q86TB8; Q8N592; B2RAQ0
• Molecular Weight: 109,195 Da
• NCBI Official Full Name: general vesicular transport factor p115 isoform 1
• NCBI Official Synonym Full Names: USO1 vesicle transport factor
• NCBI Official Symbol: USO1
• NCBI Official Synonym Symbols: TAP; VDP; P115
• NCBI Protein Information: general vesicular transport factor p115
• UniProt Protein Name: General vesicular transport factor p115
• Protein Family: Trypsinogen-like protein
• UniProt Gene Name: USO1
• UniProt Synonym Gene Names: VDP; TAP

NCBI Description

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: cytosol; endoplasmic reticulum; ER to Golgi transport vesicle membrane; fibrillar center; Golgi apparatus; Golgi membrane; Golgi stack; membrane; perinuclear region of cytoplasm; transport vesicle

Molecular Function: cadherin binding; protein binding; protein transporter activity; RNA binding

Biological Process: COPII vesicle coating; ER to Golgi vesicle-mediated transport; Golgi organization and biogenesis; Golgi vesicle docking; intracellular protein transport; transcytosis; vesicle fusion with Golgi apparatus