Human Bcl2 antagonist of cell death ELISA Kit | BAD elisa kit

Human Bcl2 antagonist of cell death (BAD) ELISA Kit

Cat. Num. AAA285699

• Gene Names: BAD; BBC2; BCL2L8
• Reactivity: Human
• Synonyms: Bcl2 antagonist of cell death; Human Bcl2 antagonist of cell death (BAD) ELISA Kit; Bcl2 Associated Death Promoter (BAD); BAD elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human BAD. No significant cross-reactivity or interference between Human BAD and analogues was observed.
• Sequence Length: 205

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for BAD elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate BAD in samples. An antibody specific for BAD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBAD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BAD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BAD bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 10835069
• NCBI GeneID: 572
• NCBI Accession #: NP_004313.1
• NCBI GenBank Nucleotide #: NM_004322.3
• UniProt Accession #: Q92934; O14803; Q6FH21
• Molecular Weight: 18,392 Da
• NCBI Official Full Name: bcl2-associated agonist of cell death
• NCBI Official Synonym Full Names: BCL2 associated agonist of cell death
• NCBI Official Symbol: BAD
• NCBI Official Synonym Symbols: BBC2; BCL2L8
• NCBI Protein Information: bcl2-associated agonist of cell death
• UniProt Protein Name: Bcl2-associated agonist of cell death
• Protein Family: Bcl2-associated agonist of cell death
• UniProt Gene Name: BAD
• UniProt Synonym Gene Names: BBC6; BCL2L8; BAD; Bcl2-L-8

NCBI Description

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytosol; mitochondrial outer membrane; mitochondrion

Molecular Function: 14-3-3 protein binding; caspase activator activity; lipid binding; phospholipid binding; protein binding; protein heterodimerization activity; protein kinase B binding; protein kinase binding; protein phosphatase 2B binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; ADP metabolic process; apoptosis; ATP metabolic process; cellular process regulating host cell cycle in response to virus; cellular response to hypoxia; cellular response to lipid; cellular response to nicotine; cerebral cortex development; cytokine-mediated signaling pathway; DNA damage response, signal transduction resulting in induction of apoptosis; glucose catabolic process; glucose homeostasis; induction of apoptosis via death domain receptors; negative regulation of cytolysis; pore complex assembly; positive regulation of apoptosis; positive regulation of apoptosis by virus; positive regulation of autophagy; positive regulation of B cell differentiation; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of epithelial cell proliferation; positive regulation of glucokinase activity; positive regulation of insulin secretion; positive regulation of intrinsic apoptotic signaling pathway; positive regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; positive regulation of mitochondrial membrane potential; positive regulation of neuron death; positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; positive regulation of proteolysis; positive regulation of release of cytochrome c from mitochondria; positive regulation of T cell differentiation; positive regulation of type B pancreatic cell development; protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; regulation of mitochondrial membrane permeability; release of cytochrome c from mitochondria; response to amino acid; response to calcium ion; response to drug; response to estradiol; response to ethanol; response to glucocorticoid stimulus; response to glucose stimulus; response to hydrogen peroxide; response to oleate; response to progesterone; response to testosterone stimulus; spermatogenesis; suppression by virus of host apoptotic process; type B pancreatic cell proliferation