Mouse Drebrin ELISA Kit | DBNL elisa kit

Mouse Drebrin-like protein (DBNL) ELISA Kit

Cat. Num. AAA285412

• Gene Names: Dbnl; Abp1; SH3P7; mAbp1
• Reactivity: Mouse
• Synonyms: Drebrin; Mouse Drebrin-like protein (DBNL) ELISA Kit; ABP1; HIP-55; SH3P7; src homology 3 domain-containing protein HIP-55; DBNL elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse DBNL. No significant cross-reactivity or interference between Mouse DBNL and analogues was observed.
• Sequence Length: 707

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DBNL elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DBNL in samples. An antibody specific for DBNL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDBNL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DBNL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DBNL bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 226423871
• NCBI GeneID: 13169
• NCBI Accession #: NP_001139780.1
• NCBI GenBank Nucleotide #: NM_001146308.1
• UniProt Accession #: Q62418; Q3TG34; Q3U5X3; Q3U8I5; Q3UZ33; Q5NCI5; Q5NCI6; Q5NCI7; Q80WP1; Q8BH56
• Molecular Weight: 48,341 Da
• NCBI Official Full Name: drebrin-like protein isoform 1
• NCBI Official Synonym Full Names: drebrin-like
• NCBI Official Symbol: Dbnl
• NCBI Official Synonym Symbols: Abp1; SH3P7; mAbp1
• NCBI Protein Information: drebrin-like protein
• UniProt Protein Name: Drebrin-like protein
• Protein Family: Drebrin
• UniProt Gene Name: Dbnl

Uniprot Description

DBNL: an SH3-containing adaptor protein. Interacts with hematopoietic progenitor kinase 1a (HIPK1). It is recruited to glycolipid-enriched microdomains following T cell receptor (TCR) stimulation. Interacts with and is phosphorylated by ZAP-70 after TCR signaling. May regulate JNK activation following TCR signaling, presumably via its interaction with HIPKIt is a member of the drebrin family of proteins that are developmentally regulated in the brain.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11 A1|11 3.87 cM

Cellular Component: cell cortex; cell junction; cell projection; cytoplasm; cytoplasmic vesicle; cytoskeleton; dendrite; endosome; extracellular exosome; Golgi apparatus; intracellular; lamellipodium; membrane; plasma membrane; podosome; postsynaptic density; ruffle; synapse

Molecular Function: actin binding; actin filament binding; cadherin binding; protein binding; protein C-terminus binding; protein domain specific binding

Biological Process: actin filament bundle formation; actin filament severing; adaptive immune response; barbed-end actin filament capping; endocytosis; immune system process; neuron projection morphogenesis; podosome assembly; Rac protein signal transduction; receptor-mediated endocytosis; synaptogenesis; transport