Human Myelin basic protein antibody (MBP-Ab) ELISA Kit | MBP-Ab elisa kit

Human Myelin basic protein antibody (MBP-Ab) ELISA Kit

Cat. Num. AAA285142

• Gene Names: MPZ; P0; CHM; DSS; MPP; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; HMSNIB
• Reactivity: Human
• Synonyms: Myelin basic protein antibody (MBP-Ab); Human Myelin basic protein antibody (MBP-Ab) ELISA Kit; MBP-Ab elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MBP-Ab. No significant cross-reactivity or interference between Human MBP-Ab and analogues was observed.
• Sequence Length: 248

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Competitive ELISA
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MBP-Ab elisa kit

Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MBP-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated MBP-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled MBP-Ab and unlabeled MBP-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of MBP-Ab in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 295391071
• NCBI GeneID: 4359
• NCBI Accession #: NP_000521.2
• NCBI GenBank Nucleotide #: NM_000530.7
• Molecular Weight: 34,387 Da
• NCBI Official Full Name: myelin protein P0 isoform MPZ
• NCBI Official Synonym Full Names: myelin protein zero
• NCBI Official Symbol: MPZ
• NCBI Official Synonym Symbols: P0; CHM; DSS; MPP; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; HMSNIB
• NCBI Protein Information: myelin protein P0
• UniProt Protein Name: Myelin protein P0
• UniProt Gene Name: MPZ
• UniProt Synonym Gene Names: MPP
• UniProt Entry Name: MYP0_HUMAN

NCBI Description

This gene is specifically expressed in Schwann cells of the peripheral nervous system and encodes a type I transmembrane glycoprotein that is a major structural protein of the peripheral myelin sheath. The encoded protein contains a large hydrophobic extracellular domain and a smaller basic intracellular domain, which are essential for the formation and stabilization of the multilamellar structure of the compact myelin. Mutations in this gene are associated with autosomal dominant form of Charcot-Marie-Tooth disease type 1 (CMT1B) and other polyneuropathies, such as Dejerine-Sottas syndrome (DSS) and congenital hypomyelinating neuropathy (CHN). A recent study showed that two isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2015]

Uniprot Description

myelin P0: a structural protein in peripheral nervous system Schwann cells. Forms an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. Defects cause of Charcot-Marie-Tooth disease, Dejerine-Sottas syndrome, congenital hypomyelination neuropathy, and Roussy-Levy syndrome.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: basolateral plasma membrane; integral to plasma membrane; lysosome; myelin sheath; plasma membrane; rough endoplasmic reticulum

Molecular Function: structural molecule activity

Biological Process: intercellular junction maintenance; negative regulation of apoptosis; synaptic transmission

Disease: Charcot-marie-tooth Disease, Axonal, Type 2i; Charcot-marie-tooth Disease, Axonal, Type 2j; Charcot-marie-tooth Disease, Demyelinating, Type 1b; Charcot-marie-tooth Disease, Dominant Intermediate D; Hypertrophic Neuropathy Of Dejerine-sottas; Neuropathy, Congenital Hypomyelinating Or Amyelinating, Autosomal Recessive; Roussy-levy Hereditary Areflexic Dystasia