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Human Thyroid Stimulating Hormone ELISA Kit | TSH elisa kit

Human Thyroid Stimulating Hormone (TSH) ELISA Kit

Gene Names
CGA; HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA
Reactivity
Human
Synonyms
Thyroid Stimulating Hormone; Human Thyroid Stimulating Hormone (TSH) ELISA Kit; TSH elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human TSH. No significant cross-reactivity or interference between Human TSH and analogues was observed.
Sequence Length
116
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for TSH elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TSH in samples. An antibody specific for TSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
13,075 Da
NCBI Official Full Name
thyroid-stimulating hormone alpha subunit
NCBI Official Synonym Full Names
glycoprotein hormones, alpha polypeptide
NCBI Official Symbol
CGA
NCBI Official Synonym Symbols
HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA
NCBI Protein Information
glycoprotein hormones alpha chain
UniProt Protein Name
Glycoprotein hormones alpha chain
UniProt Gene Name
CGA
UniProt Synonym Gene Names
CG-alpha; FSH-alpha; LSH-alpha; TSH-alpha
UniProt Entry Name
GLHA_HUMAN

NCBI Description

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CGA: The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q12-q21

Cellular Component: extracellular region; Golgi lumen

Molecular Function: hormone activity; protein binding

Biological Process: cell-cell signaling; cellular protein metabolic process; peptide hormone processing; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; signal transduction

Research Articles on TSH

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Product Notes

The Human TSH cga (Catalog #AAA284568) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284568 ELISA Kit recognizes Human TSH. It is sometimes possible for the material contained within the vial of "Thyroid Stimulating Hormone, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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