Mouse Protein 4.1 ELISA Kit | EPB41 elisa kit

Mouse Protein 4.1 (EPB41) ELISA Kit

Cat. Num. AAA283822

• Gene Names: Epb41; 4.1R; Elp1; Elp-1; Epb4.1; AI415518; mKIAA4056; D4Ertd442e
• Reactivity: Mouse
• Synonyms: Protein 4.1; Mouse Protein 4.1 (EPB41) ELISA Kit; RP11-242O24.1; 4.1R; EL1; HE; erythrocyte membrane protein band 4.1|erythrocyte surface protein band 4.1; EPB41 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse EPB41. No significant cross-reactivity or interference between Mouse EPB41 and analogues was observed.
• Sequence Length: 601

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for EPB41 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EPB41 in samples. An antibody specific for EPB41 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEPB41 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPB41 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPB41 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 190881478
• NCBI GeneID: 269587
• NCBI Accession #: NP_001122078.1
• NCBI GenBank Nucleotide #: NM_001128606.1
• UniProt Accession #: P48193; Q5DTQ8; Q68FF1; Q6NVF5; A2A843
• Molecular Weight: 89,664 Da
• NCBI Official Full Name: protein 4.1 isoform 2
• NCBI Official Synonym Full Names: erythrocyte membrane protein band 4.1
• NCBI Official Symbol: Epb41
• NCBI Official Synonym Symbols: 4.1R; Elp1; Elp-1; Epb4.1; AI415518; mKIAA4056; D4Ertd442e
• NCBI Protein Information: protein 4.1
• UniProt Protein Name: Protein 4.1
• Protein Family: Protein
• UniProt Gene Name: Epb41
• UniProt Synonym Gene Names: Epb4.1; Kiaa4056; P4.1

Uniprot Description

EPB41: Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Defects in EPB41 are the cause of elliptocytosis type 1 (EL1). EL1 is a Rhesus-linked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant, hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in EPB41 are a cause of hereditary pyropoikilocytosis (HPP). HPP is an autosomal recessive hematologic disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 4 D2.3|4 64.54 cM

Cellular Component: actin cytoskeleton; cell junction; cortical cytoskeleton; cytoplasm; cytoplasmic side of plasma membrane; cytoskeleton; cytosol; membrane; nucleus; plasma membrane; postsynaptic density; protein complex

Molecular Function: actin binding; calmodulin binding; cytoskeletal protein binding; phosphatidylinositol binding; phosphoprotein binding; protein C-terminus binding; protein N-terminus binding; spectrin binding; structural constituent of cytoskeleton; structural molecule activity

Biological Process: actin cytoskeleton organization; actomyosin structure organization; cell cycle; cell division; cortical actin cytoskeleton organization and biogenesis; positive regulation of protein binding; positive regulation of protein localization to cell cortex; protein complex assembly; regulation of cell shape; transport