Sheep Flap endonuclease 1 ELISA Kit | FEN1 elisa kit

Sheep Flap endonuclease 1 (FEN1) ELISA Kit

Cat. Num. AAA283769

• Gene Names: FEN1; FEN-1
• Reactivity: Sheep
• Synonyms: Flap endonuclease 1; Sheep Flap endonuclease 1 (FEN1) ELISA Kit; FEN-1; MF1; RAD2; DNase IV|maturation factor-1; FEN1 elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sheep FEN1. No significant cross-reactivity or interference between Sheep FEN1 and analogues was observed.
• Sequence Length: 380

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for FEN1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FEN1 in samples. An antibody specific for FEN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFEN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FEN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FEN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 261244970
• NCBI GeneID: 100307042
• NCBI Accession #: NP_001159668.1
• NCBI GenBank Nucleotide #: NM_001166196.1
• UniProt Accession #: C8BKD0
• Molecular Weight: 42,676 Da
• NCBI Official Full Name: flap endonuclease 1
• NCBI Official Symbol: FEN1
• NCBI Official Synonym Symbols: FEN-1
• NCBI Protein Information: flap endonuclease 1
• UniProt Protein Name: Flap endonuclease 1
• Protein Family: Flap endonuclease
• UniProt Gene Name: FEN1
• UniProt Synonym Gene Names: FEN-1

Uniprot Description

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.