Porcine Chemerin ELISA Kit | CHE elisa kit

Porcine Chemerin ELISA Kit

Cat. Num. AAA28355

• Gene Names: CMKLR1; DEZ; RVER1; ChemR23; CHEMERINR
• Reactivity: Porcine
• Synonyms: Chemerin; Porcine Chemerin ELISA Kit; CHE elisa kit

• Reactivity: Porcine
• Specificity: No significant cross-reactivity or interference between Porcine CHE and analogues was observed.
• Sequence Length: 373

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2ng/ml-1000ng/ml.
• Sensitivity: 5.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CHE concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine CHE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CHE and CHE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CHE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for CHE elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CHE concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine CHE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CHE and CHE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CHE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 215272321
• NCBI GeneID: 1240
• NCBI Accession #: NP_001135817.1
• NCBI GenBank Nucleotide #: NM_001142345.1
• UniProt Accession #: Q99788; O75748; Q3KP37; Q5U0H0; Q99789; A8K6Y5
• Molecular Weight: 42,322 Da
• NCBI Official Full Name: chemokine-like receptor 1 isoform a
• NCBI Official Synonym Full Names: chemokine-like receptor 1
• NCBI Official Symbol: CMKLR1
• NCBI Official Synonym Symbols: DEZ; RVER1; ChemR23; CHEMERINR
• NCBI Protein Information: chemokine-like receptor 1; chemerin receptor; resolvin E1 receptor; chemokine receptor-like 1; G-protein coupled receptor DEZ; G-protein coupled receptor ChemR23; orphan G-protein coupled receptor, Dez
• UniProt Protein Name: Chemokine-like receptor 1
• UniProt Gene Name: CMKLR1
• UniProt Synonym Gene Names: CHEMR23; DEZ
• UniProt Entry Name: CML1_HUMAN

Uniprot Description

CMKLR1: Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF- kappa-B. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2). Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; chemokine receptor activity; receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of interleukin-12 production; inhibition of NF-kappaB transcription factor; positive regulation of fat cell differentiation; regulation of calcium-mediated signaling; immune response; chemotaxis; skeletal development