Human Probable histone-lysine N-methyltransferase ELISA Kit | ASH1L elisa kit
Human Probable histone-lysine N-methyltransferase (ASH1L) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ASH1L in Human serum, plasma. An antibody specific for ASH1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ASH1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASH1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASH1L bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. [provided by RefSeq, Jul 2008]
Uniprot Description
ASH1L: a protein methyltransferase that specifically methylates K36 of histone H3 (H3K36me). A member of the trithorax group (trxG) of transcriptional activators that is required for proper expression of Hox genes and is preferentially expressed in haematopoietic stem cells. Widely expressed, with high levels in brain, heart and kidney. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions.
Protein type: Amino Acid Metabolism - lysine degradation; Cell adhesion; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: bicellular tight junction; chromosome; extracellular exosome; Golgi apparatus; nucleoplasm; nucleus
Molecular Function: chromatin binding; DNA binding; histone methyltransferase activity (H3-K36 specific); histone methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; metal ion binding
Biological Process: cell-cell signaling; decidualization; DNA packaging; histone H3-K4 methylation; interleukin-6 production; negative regulation of acute inflammatory response; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; single fertilization; skeletal system development; transcription from RNA polymerase II promoter
Disease: Mental Retardation, Autosomal Dominant 52
Research Articles on ASH1L
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Product Notes
The Human ASH1L ash1l (Catalog #AAA283034) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283034 ELISA Kit recognizes Human ASH1L. It is sometimes possible for the material contained within the vial of "Probable histone-lysine N-methyltransferase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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