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Rat Acyl-coenzyme A thioesterase 1 ELISA Kit | ACOT1 elisa kit

Rat Acyl-coenzyme A thioesterase 1 (ACOT1) ELISA Kit

Gene Names
Acot1; Cte1
Reactivity
Rat
Synonyms
Acyl-coenzyme A thioesterase 1; Rat Acyl-coenzyme A thioesterase 1 (ACOT1) ELISA Kit; ACOT1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat ACOT1. No significant cross-reactivity or interference between Rat ACOT1 and analogues was observed.
Sequence Length
421
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ACOT1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ACOT1 in samples. An antibody specific for ACOT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACOT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACOT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACOT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,013 Da
NCBI Official Full Name
acyl-coenzyme A thioesterase 1
NCBI Official Synonym Full Names
acyl-CoA thioesterase 1
NCBI Official Symbol
Acot1
NCBI Official Synonym Symbols
Cte1
NCBI Protein Information
acyl-coenzyme A thioesterase 1
UniProt Protein Name
Acyl-coenzyme A thioesterase 1
UniProt Gene Name
Acot1
UniProt Synonym Gene Names
Cte1; Acyl-CoA thioesterase 1; ACH2; Long chain acyl-CoA hydrolase

NCBI Description

mouse homolog has acyl-CoA thioesterase activity against long-chain acyl-CoAs; may play a role in lipid metabolism [RGD, Feb 2006]

Uniprot Description

ACOT1: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16. Belongs to the C/M/P thioester hydrolase family.

Protein type: EC 3.1.2.2; Hydrolase; Lipid Metabolism - unsaturated fatty acid biosynthesis

Chromosomal Location of Human Ortholog: 6q24

Cellular Component: cytoplasm; cytosol

Molecular Function: acyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activity

Biological Process: acyl-CoA metabolic process; fatty acid metabolic process; lipid metabolic process

Research Articles on ACOT1

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Product Notes

The Rat ACOT1 acot1 (Catalog #AAA282962) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282962 ELISA Kit recognizes Rat ACOT1. It is sometimes possible for the material contained within the vial of "Acyl-coenzyme A thioesterase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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