Human Putative hydrolase RBBP9 ELISA Kit | RBBP9 elisa kit
Human Putative hydrolase RBBP9 (RBBP9) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RBBP9 in samples. An antibody specific for RBBP9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBBP9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBBP9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBBP9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. Two alternatively spliced transcript variants of this gene with identical predicted protein products have been reported, one of which is a nonsense-mediated decay candidate. [provided by RefSeq, Jul 2008]
Uniprot Description
RBBP9: May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. Belongs to the RBBP9 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.-.-.-; Hydrolase
Chromosomal Location of Human Ortholog: 20p11.23
Cellular Component: cytoplasm; nucleoplasm
Molecular Function: hydrolase activity; protein binding
Biological Process: regulation of cell proliferation
Research Articles on RBBP9
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Product Notes
The Human RBBP9 rbbp9 (Catalog #AAA282893) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282893 ELISA Kit recognizes Human RBBP9. It is sometimes possible for the material contained within the vial of "Putative hydrolase RBBP9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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