Human Probable ATP-dependent RNA helicase DDX41 ELISA Kit | DDX41 elisa kit

Human Probable ATP-dependent RNA helicase DDX41 (DDX41) ELISA Kit

Cat. Num. AAA282814

• Gene Names: DDX41; ABS; MPLPF
• Reactivity: Human
• Synonyms: Probable ATP-dependent RNA helicase DDX41; Human Probable ATP-dependent RNA helicase DDX41 (DDX41) ELISA Kit; DDX41 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human DDX41. No significant cross-reactivity or interference between Human DDX41 and analogues was observed.
• Sequence Length: 622

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DDX41 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DDX41 in samples. An antibody specific for DDX41 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDX41 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDX41 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDX41 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 1012431804
• NCBI GeneID: 51428
• NCBI Accession #: NP_001308661.1
• NCBI GenBank Nucleotide #: NM_001321732.1
• UniProt Accession #: Q9UJV9; Q96BK6; Q96K05; Q9NT96; Q9NW04; B2RDC8
• Molecular Weight: 69,838 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX41 isoform 2
• NCBI Official Synonym Full Names: DEAD-box helicase 41
• NCBI Official Symbol: DDX41
• NCBI Official Synonym Symbols: ABS; MPLPF
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX41
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX41
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX41
• UniProt Synonym Gene Names: ABS

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD box protein family and interacts with several spliceosomal proteins. In addition, the encoded protein may recognize the bacterial second messengers cyclic di-GMP and cyclic di-AMP, resulting in the induction of genes involved in the innate immune response. [provided by RefSeq, Jan 2017]

Uniprot Description

DDX41: Probable ATP-dependent RNA helicase. Is required during post-transcriptional gene expression. May be involved in pre-mRNA splicing. Belongs to the DEAD box helicase family. DDX41 subfamily.

Protein type: EC 3.6.4.13; Helicase; RNA splicing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: catalytic step 2 spliceosome; cytoplasm; cytosol; endoplasmic reticulum; membrane; nucleolus; nucleus; spliceosomal complex

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; DNA binding; metal ion binding; protein binding; RNA binding

Biological Process: apoptosis; cell differentiation; cell proliferation; defense response to virus; mRNA splicing, via spliceosome; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; regulation of type I interferon production; RNA secondary structure unwinding

Disease: Myeloproliferative/lymphoproliferative Neoplasms, Familial (multiple Types), Susceptibility To