Human Pre-B lymphocyte protein 3 ELISA Kit | VPREB3 elisa kit

Human Pre-B lymphocyte protein 3 (VPREB3) ELISA Kit

Cat. Num. AAA282431

• Gene Names: VPREB3; 8HS20; N27C7-2
• Reactivity: Human
• Synonyms: Pre-B lymphocyte protein 3; Human Pre-B lymphocyte protein 3 (VPREB3) ELISA Kit; VPREB3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human VPREB3. No significant cross-reactivity or interference between Human VPREB3 and analogues was observed.
• Sequence Length: 123

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for VPREB3 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate VPREB3 in samples. An antibody specific for VPREB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVPREB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VPREB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VPREB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 7019567
• NCBI GeneID: 29802
• NCBI Accession #: NP_037510.1
• NCBI GenBank Nucleotide #: NM_013378.2
• UniProt Accession #: Q9UKI3; B2R587
• Molecular Weight: 13,710 Da
• NCBI Official Full Name: pre-B lymphocyte protein 3
• NCBI Official Synonym Full Names: V-set pre-B cell surrogate light chain 3
• NCBI Official Symbol: VPREB3
• NCBI Official Synonym Symbols: 8HS20; N27C7-2
• NCBI Protein Information: pre-B lymphocyte protein 3
• UniProt Protein Name: Pre-B lymphocyte protein 3
• Protein Family: Pre-B lymphocyte protein
• UniProt Gene Name: VPREB3

NCBI Description

The protein encoded by this gene is the human ortholog of the mouse VpreB3 (8HS20) protein, is thought to be involved in B-cell maturation, and may play a role in assembly of the pre-B cell receptor (pre-BCR). While the role of this protein in B-cell development has not yet been elucidated, studies with the chicken ortholog of this protein have found that when overexpressed, this protein localizes to the endoplasmic reticulum. The mouse ortholog of this protein has been shown to associate with membrane mu heavy chains early in the course of pre-B cell receptor biosynthesis. Expression of this gene has been observed in some lymphomas. [provided by RefSeq, Apr 2015]

Uniprot Description

VPREB3: Associates with the Ig-mu chain to form a molecular complex that is expressed on the surface of pre-B-cells. Belongs to the immunoglobulin superfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q11.23|22q11

Cellular Component: endoplasmic reticulum; extracellular region; extracellular space

Biological Process: immune response; immunoglobulin production; leukocyte migration