Bovine 11-cis retinol dehydrogenase ELISA Kit | RDH5 elisa kit
Bovine 11-cis retinol dehydrogenase (RDH5) ELISA Kit
Gene Names
RDH5; P32; 9cRDH
Reactivity
Bovine
Synonyms
11-cis retinol dehydrogenase; Bovine 11-cis retinol dehydrogenase (RDH5) ELISA Kit; RDH5 elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine RDH5. No significant cross-reactivity or interference between Bovine RDH5 and analogues was observed.
Sequence Length
318
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for RDH5 elisa kit
RDH5 encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments.
The sequence of the cDNA encoding p32 revealed that it is a member of the superfamily of short-chain alcohol dehydrogenases. The authors determined that transcripts of the gene were specifically expressed in retinal pigment epithelium. They also determined that p32 acts as a stereospecific 11-cis retinol dehydrogenase in the presence of NAD+ and that it catalyzes the final step in the biosynthesis of 11-cis retinaldehyde, the universal chromophore of visual pigments.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RDH5 in samples. An antibody specific for RDH5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRDH5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RDH5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RDH5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
The sequence of the cDNA encoding p32 revealed that it is a member of the superfamily of short-chain alcohol dehydrogenases. The authors determined that transcripts of the gene were specifically expressed in retinal pigment epithelium. They also determined that p32 acts as a stereospecific 11-cis retinol dehydrogenase in the presence of NAD+ and that it catalyzes the final step in the biosynthesis of 11-cis retinaldehyde, the universal chromophore of visual pigments.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RDH5 in samples. An antibody specific for RDH5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRDH5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RDH5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RDH5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,037 Da
NCBI Official Full Name
11-cis retinol dehydrogenase
NCBI Official Synonym Full Names
retinol dehydrogenase 5
NCBI Official Symbol
RDH5
NCBI Official Synonym Symbols
P32; 9cRDH
NCBI Protein Information
11-cis retinol dehydrogenase
UniProt Protein Name
11-cis retinol dehydrogenase
Protein Family
UniProt Gene Name
RDH5
UniProt Synonym Gene Names
RDH1; 11-cis RDH; 11-cis RoDH; 9cRDH
Uniprot Description
Stereospecific 11-cis retinol dehydrogenase, which catalyzes the final step in the biosynthesis of 11-cis retinaldehyde, the universal chromophore of visual pigments. Also able to oxidize 9-cis-retinol and 13-cis-retinol, but not all-trans-retinol. Active in the presence of NAD as cofactor but not in the presence of NADP.
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Product Notes
The Bovine RDH5 rdh5 (Catalog #AAA281672) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281672 ELISA Kit recognizes Bovine RDH5. It is sometimes possible for the material contained within the vial of "11-cis retinol dehydrogenase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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