Human Phosphoenolpyruvate carboxykinase [GTP] ELISA Kit | PCK2 elisa kit
Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PCK2 in Human serum, plasma, tissue homogenates. An antibody specific for PCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]
Uniprot Description
PCK2: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Monomer. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; EC 4.1.1.32; Kinase, other; Lyase; Mitochondrial
Chromosomal Location of Human Ortholog: 14q11.2-q12
Cellular Component: mitochondrial matrix; mitochondrion
Molecular Function: GTP binding; metal ion binding; phosphoenolpyruvate carboxykinase (GTP) activity; phosphoenolpyruvate carboxykinase activity; protein binding
Biological Process: gluconeogenesis; NADH oxidation; oxaloacetate metabolic process; positive regulation of insulin secretion; pyruvate metabolic process; response to lipopolysaccharide
Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Mitochondrial
Research Articles on PCK2
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Product Notes
The Human PCK2 pck2 (Catalog #AAA281601) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281601 ELISA Kit recognizes Human PCK2. It is sometimes possible for the material contained within the vial of "Phosphoenolpyruvate carboxykinase [GTP], ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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