Mouse Zinc finger and BTB domain-containing protein 17 ELISA Kit | ZBTB17 elisa kit
Mouse Zinc finger and BTB domain-containing protein 17 (ZBTB17) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZBTB17 in samples. An antibody specific for ZBTB17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZBTB17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZBTB17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZBTB17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
Uniprot Description
ZBTB17: a transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation. Represses RB1 transcription; this repression can be blocked by interaction with ZBTB49 isoform 3/ZNF509S1. Belongs to the krueppel C2H2-type zinc-finger protein family. Binds to the C-terminal helix-loop-helix motif of MYC which inhibits ZBTB17 transactivation and growth arrest activities and renders it insoluble in the nucleus. Also interacts with HCFC1, MAGEA4 and TMPRSS11A. Three isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 4|4 D3
Cellular Component: nucleus; protein complex
Molecular Function: DNA binding; DNA binding transcription factor activity; metal ion binding; nucleic acid binding; protein binding; transcription factor binding
Biological Process: ectoderm development; gastrulation with mouth forming second; multicellular organism development; negative regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription, DNA-templated; transcription, DNA-dependent
Research Articles on ZBTB17
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Product Notes
The Mouse ZBTB17 zbtb17 (Catalog #AAA281321) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281321 ELISA Kit recognizes Mouse ZBTB17. It is sometimes possible for the material contained within the vial of "Zinc finger and BTB domain-containing protein 17, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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