Human Probable ATP-dependent RNA helicase DDX53 ELISA Kit | DDX53 elisa kit
Human Probable ATP-dependent RNA helicase DDX53 (DDX53) ELISA Kit
Gene Names
DDX53; CAGE; CT26
Reactivity
Human
Synonyms
Probable ATP-dependent RNA helicase DDX53; Human Probable ATP-dependent RNA helicase DDX53 (DDX53) ELISA Kit; DDX53 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human SLC26A5. No significant cross-reactivity or interference between Human SLC26A5 and analogues was observed.
Sequence Length
631
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
7.8 pg/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for DDX53 elisa kit
Intended Uses: For the quantitative detection of Human Prestin (SLC26A5) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SLC26A5 in Human serum, plasma. An antibody specific for SLC26A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC26A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC26A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC26A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SLC26A5 in Human serum, plasma. An antibody specific for SLC26A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC26A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC26A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC26A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,154 Da
NCBI Official Full Name
DEAD box protein 53
NCBI Official Synonym Full Names
DEAD-box helicase 53
NCBI Official Symbol
DDX53
NCBI Official Synonym Symbols
CAGE; CT26
NCBI Protein Information
DEAD box protein 53
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX53
Protein Family
UniProt Gene Name
DDX53
UniProt Synonym Gene Names
CAGE; CT26
NCBI Description
This intronless gene encodes a protein which contains several domains found in members of the DEAD-box helicase protein family. Other members of this protein family participate in ATP-dependent RNA unwinding. [provided by RefSeq, Sep 2011]
Uniprot Description
DDX53: This intronless gene encodes a protein which contains several domains found in members of the DEAD-box helicase protein family. Other members of this protein family participate in ATP-dependent RNA unwinding. [provided by RefSeq, Sep 2011]
Protein type: Cancer Testis Antigen (CTA); EC 3.6.4.13; Helicase
Chromosomal Location of Human Ortholog: Xp22.11
Cellular Component: cytoplasm; nucleolus
Molecular Function: ATP binding; ATP-dependent RNA helicase activity; RNA binding
Biological Process: RNA secondary structure unwinding
Research Articles on DDX53
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Product Notes
The Human DDX53 ddx53 (Catalog #AAA281305) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281305 ELISA Kit recognizes Human DDX53. It is sometimes possible for the material contained within the vial of "Probable ATP-dependent RNA helicase DDX53, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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