Human Permeability glycoprotein ELISA Kit | P-gp elisa kit

Human Permeability glycoprotein (P-gp) ELISA Kit

Cat. Num. AAA281012

• Gene Names: ABCB1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
• Reactivity: Human
• Synonyms: Permeability glycoprotein; Human Permeability glycoprotein (P-gp) ELISA Kit; P-gp elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ABCB1. No significant cross-reactivity or interference between Human ABCB1 and analogues was observed.
• Sequence Length: 832

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for P-gp elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ABCB1 in samples. An antibody specific for ABCB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 42741659
• NCBI GeneID: 5243
• NCBI Accession #: NP_000918.2
• NCBI GenBank Nucleotide #: NM_000927.4
• UniProt Accession #: P08183; Q12755; Q14812; A8K294; B5AK60
• Molecular Weight: 134,032 Da
• NCBI Official Full Name: multidrug resistance protein 1 isoform 2
• NCBI Official Synonym Full Names: ATP binding cassette subfamily B member 1
• NCBI Official Symbol: ABCB1
• NCBI Official Synonym Symbols: CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
• NCBI Protein Information: multidrug resistance protein 1
• UniProt Protein Name: Multidrug resistance protein 1
• UniProt Gene Name: ABCB1
• UniProt Synonym Gene Names: MDR1; PGY1

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017]

Uniprot Description

ABCB1: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Interacts with PSMB8.

Protein type: Cell adhesion; EC 3.6.3.44; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 7q21.12

Cellular Component: apical plasma membrane; cell surface; extracellular exosome; integral component of membrane; membrane; plasma membrane

Molecular Function: ATP binding; ATPase activity, coupled to transmembrane movement of substances; ceramide-translocating ATPase activity; phosphatidylcholine-translocating ATPase activity; phosphatidylethanolamine-translocating ATPase activity; protein binding; transporter activity; xenobiotic transmembrane transporting ATPase activity

Biological Process: ceramide translocation; drug transmembrane transport; G2/M transition of mitotic cell cycle; phospholipid translocation; positive regulation of anion channel activity; regulation of chloride transport; regulation of response to osmotic stress; response to drug; stem cell proliferation; transmembrane transport; transport; xenobiotic transport

Disease: Colchicine Resistance; Inflammatory Bowel Disease 13