Mouse Zinc finger protein 280D ELISA Kit | ZNF280D elisa kit
Mouse Zinc finger protein 280D (ZNF280D) ELISA Kit
Gene Names
Zfp280d; Suhw4; ZNF634; Znf280d; BC027163; A930005F02Rik
Reactivity
Mouse
Synonyms
Zinc finger protein 280D; Mouse Zinc finger protein 280D (ZNF280D) ELISA Kit; ZNF280D elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZNF280D. No significant cross-reactivity or interference between Mouse ZNF280D and analogues was observed.
Sequence Length
969
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZNF280D elisa kit
The ZNF280D protein is 979 amino acids in length. The protein contains a domain of unknown function spanning from amino acid 45 to amino acid 230. DUF4195 is a family that is found at the N-terminus of metazoan proteins that carry PHD-like zinc-finger domains; the function is unknown. ZNF280D protein also contains five highly conserved Cys2His2-type zinc finger domains. Zinc fingers have the ability to bind DNA, which supports the speculative role of ZNF280D as a transcription factor. The protein has a weight of approximately 109.3 kdal. Charge cluster analysis reveals a negative charge cluster near the N-terminus from amino acids 16-43. Charge clusters are associated with functional domains of cellular transcription factors, providing further support for ZNF280D as a possible transcription factor.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF280D in samples. An antibody specific for ZNF280D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF280D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF280D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF280D bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF280D in samples. An antibody specific for ZNF280D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF280D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF280D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF280D bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,512 Da
NCBI Official Full Name
zinc finger protein 280D isoform 2
NCBI Official Synonym Full Names
zinc finger protein 280D
NCBI Official Symbol
Zfp280d
NCBI Official Synonym Symbols
Suhw4; ZNF634; Znf280d; BC027163; A930005F02Rik
NCBI Protein Information
zinc finger protein 280D
UniProt Protein Name
Zinc finger protein 280D
Protein Family
UniProt Gene Name
Znf280d
UniProt Synonym Gene Names
Suhw4; Zfp280d
Uniprot Description
ZNF280D: May function as a transcription factor. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 9|9 D
Cellular Component: nucleus
Molecular Function: DNA binding; metal ion binding; nucleic acid binding
Biological Process: regulation of transcription, DNA-templated; transcription, DNA-dependent
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Product Notes
The Mouse ZNF280D znf280d (Catalog #AAA280959) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280959 ELISA Kit recognizes Mouse ZNF280D. It is sometimes possible for the material contained within the vial of "Zinc finger protein 280D, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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