Human Probable E3 ubiquitin-protein ligase TRIM8 ELISA Kit | TRIM8 elisa kit

Human Probable E3 ubiquitin-protein ligase TRIM8 (TRIM8) ELISA Kit

Cat. Num. AAA280802

• Gene Names: TRIM8; GERP; RNF27
• Reactivity: Human
• Synonyms: Probable E3 ubiquitin-protein ligase TRIM8; Human Probable E3 ubiquitin-protein ligase TRIM8 (TRIM8) ELISA Kit; TRIM8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human TRIM8. No significant cross-reactivity or interference between Human TRIM8 and analogues was observed.
• Sequence Length: 551

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for TRIM8 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TRIM8 in samples. An antibody specific for TRIM8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRIM8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIM8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIM8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 148596968
• NCBI GeneID: 81603
• NCBI Accession #: NP_112174.2
• NCBI GenBank Nucleotide #: NM_030912.2
• UniProt Accession #: Q9BZR9; Q9C028; A6NI31
• Molecular Weight: 61,489 Da
• NCBI Official Full Name: probable E3 ubiquitin-protein ligase TRIM8 isoform 1
• NCBI Official Synonym Full Names: tripartite motif containing 8
• NCBI Official Symbol: TRIM8
• NCBI Official Synonym Symbols: GERP; RNF27
• NCBI Protein Information: probable E3 ubiquitin-protein ligase TRIM8
• UniProt Protein Name: Probable E3 ubiquitin-protein ligase TRIM8
• Protein Family: Probable E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM8
• UniProt Synonym Gene Names: GERP; RNF27

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) protein family. Based on similarities to other proteins, the encoded protein is suspected to be an E3 ubiquitin-protein ligase. Regulation of this gene may be altered in some cancers. Mutations resulting in a truncated protein product have been observed in early-onset epileptic encephalopathy (EOEE). [provided by RefSeq, Sep 2016]

Uniprot Description

TRIM8: Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1. Belongs to the TRIM/RBCC family.

Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: cytosol; PML body

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; transferase activity; zinc ion binding

Biological Process: innate immune response; negative regulation of viral transcription; negative regulation of virion penetration into host cell; positive regulation of autophagy; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of NF-kappaB transcription factor activity; positive regulation of transcription factor activity; protein ubiquitination; stem cell maintenance