Mouse Y+L amino acid transporter 1 ELISA Kit | SLC7A7 elisa kit

Mouse Y+L amino acid transporter 1 (SLC7A7) ELISA Kit

Cat. Num. AAA280633

• Gene Names: Slc7a7; my+lat1; AI790233
• Reactivity: Mouse
• Synonyms: Y+L amino acid transporter 1; Mouse Y+L amino acid transporter 1 (SLC7A7) ELISA Kit; SLC7A7 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse SLC7A7. No significant cross-reactivity or interference between Mouse SLC7A7 and analogues was observed.
• Sequence Length: 511

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for SLC7A7 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SLC7A7 in samples. An antibody specific for SLC7A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 358439451
• NCBI GeneID: 20540
• NCBI Accession #: NP_001240608.1
• NCBI GenBank Nucleotide #: NM_001253679.1
• UniProt Accession #: Q9Z1K8; Q9QWS1
• Molecular Weight: 55,677 Da
• NCBI Official Full Name: Y+L amino acid transporter 1
• NCBI Official Synonym Full Names: solute carrier family 7 (cationic amino acid transporter, y+ system), member 7
• NCBI Official Symbol: Slc7a7
• NCBI Official Synonym Symbols: my+lat1; AI790233
• NCBI Protein Information: Y+L amino acid transporter 1
• UniProt Protein Name: Y+L amino acid transporter 1
• Protein Family: Y+L amino acid transporter
• UniProt Gene Name: Slc7a7
• UniProt Synonym Gene Names: Y+LAT1; y+LAT-1

Uniprot Description

SLC7A7: Involved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Involved in the transport of L- arginine in monocytes. Defects in SLC7A7 are the cause of lysinuric protein intolerance (LPI). LPI is an autosomal recessive multisystem disorder found mainly in Finland and Italy. On a normal diet, LPI patients present poor feeding, vomiting, diarrhea, episodes of hyperammoniaemic coma and growth retardation. Hepatosplenomegaly, osteoporosis and a life- threatening pulmonary involvement (alveolar proteinosis) are also seen. Biochemically LPI is characterized by a defect in the plasma membrane transport of dibasic amino acids. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 14|14 C2

Cellular Component: integral component of membrane; integral component of plasma membrane; membrane; plasma membrane

Molecular Function: amino acid transmembrane transporter activity; antiporter activity; basic amino acid transmembrane transporter activity; L-amino acid transmembrane transporter activity

Biological Process: amino acid transport; regulation of arginine metabolic process; transport