Human Probable ATP-dependent RNA helicase DDX60-like ELISA Kit | DDX60L elisa kit

Human Probable ATP-dependent RNA helicase DDX60-like (DDX60L) ELISA Kit

Cat. Num. AAA280485

• Reactivity: Human
• Synonyms: Probable ATP-dependent RNA helicase DDX60-like; Human Probable ATP-dependent RNA helicase DDX60-like (DDX60L) ELISA Kit; DDX60L elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human DDX60L. No significant cross-reactivity or interference between Human DDX60L and analogues was observed.
• Sequence Length: 1706

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DDX60L elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DDX60L in samples. An antibody specific for DDX60L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDX60L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDX60L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDX60L bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 613410189
• NCBI GeneID: 91351
• NCBI Accession #: NP_001012985.2
• NCBI GenBank Nucleotide #: NM_001012967.2
• UniProt Accession #: Q5H9U9; Q96ND6
• Molecular Weight: 152,253 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX60-like isoform 1
• NCBI Official Synonym Full Names: DExD/H-box 60 like
• NCBI Official Symbol: DDX60L
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX60-like
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX60-like
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX60L

NCBI Description

This gene encodes a member of the DExD/H-box helicase family of proteins, a subset of the super family 2 helicases. Members of the DExD/H-box helicase family share a conserved functional core comprised of two RecA-like globular domains. These domains contain conserved motifs that mediate ATP binding, ATP hydrolysis, nucleic acid binding, and RNA unwinding. In addition to functions in RNA metabolism, members of this family are involved in anti-viral immunity and act as cytosolic sensors of viral nucleic acids. The protein encoded by this gene has been shown to inhibit hepatitis C virus replication in response to interferon stimulation in cell culture. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

Uniprot Description

DDX60L: Belongs to the helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: 4q32.3

Molecular Function: ATP binding; helicase activity; RNA binding