Sheep Heat shock protein beta-1 (HSPB1) ELISA Kit | HSPB1 elisa kit

Sheep Heat shock protein beta-1 (HSPB1) ELISA Kit

Cat. Num. AAA2803310

• Reactivity: Sheep
• Synonyms: Heat shock protein beta-1 (HSPB1); Sheep Heat shock protein beta-1 (HSPB1) ELISA Kit; CMT2F; DKFZp586P1322; HMN2B; HS.76067; HSP27; HSP28; Hsp25; SRP27; estrogen-regulated 24kDa protein|heat shock 27kD protein 1|heat shock protein beta-1|stress-responsive protein 27; HSPB1 elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sheep HSPB1. No significant cross-reactivity or interference between Sheep HSPB1 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate HSPB1 in samples. An antibody specific for HSPB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

NCBI and Uniprot Product Information

• NCBI GI #: 45384222
• NCBI GeneID: 396227
• NCBI Accession #: NP_990621.1
• NCBI GenBank Nucleotide #: NM_205290.1
• UniProt Accession #: Q00649
• Molecular Weight: 21,672 Da
• NCBI Official Full Name: heat shock protein beta-1
• NCBI Official Synonym Full Names: heat shock 27kDa protein 1
• NCBI Official Symbol: HSPB1
• NCBI Protein Information: heat shock protein beta-1; HSP 25; HSP 27; 25 kDa IAP; heat shock 25 kDa protein; heat shock 27 kDa protein; actin polymerization inhibitor; inhibitor of actin polymerization
• UniProt Protein Name: Heat shock protein beta-1
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HspB1; HSP 25; HSP 27
• UniProt Entry Name: HSPB1_CHICK

Uniprot Description

Function: Markedly reduces low-shear viscosity of F-actin and suppresses the rate of polymerization in vitro. May behave as a barbed-end capping protein devoid of nucleating activity.