Human PH-interacting protein (PHIP) ELISA Kit | PHIP elisa kit

Human PH-interacting protein (PHIP) ELISA Kit

Cat. Num. AAA2802283

• Gene Names: PHIP; ndrp; BRWD2; WDR11; DCAF14
• Reactivity: Human
• Synonyms: PH-interacting protein (PHIP); Human PH-interacting protein (PHIP) ELISA Kit; RP11-173D14.2; DCAF14; FLJ20705; FLJ45918; MGC90216; WDR11; ndrp; DDB1 and CUL4 associated factor 14; PHIP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PHIP. No significant cross-reactivity or interference between Human PHIP and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate PHIP in samples. An antibody specific for PHIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

Related Product Information for PHIP elisa kit

PHIP binds the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS1), modulates insulin signaling, and plays a role in pancreatic beta cell growth and survival.
The deduced 902-amino acid mouse and human proteins have a calculated molecular mass of 100 kD. Both have 2 predicted bromodomains that share 44% identity with the bromodomain of mouse CREB-binding protein (CREBBP). Northern blot analysis of adult mouse tissues detected a 7.0-kb transcript with widespread expression. Western blot analysis of human myeloma cell lysates detected a long 104-kD isoform and a shorter 97-kD isoform, which were predicted to arise from alternative usage of 2 putative translation initiation sites.

NCBI and Uniprot Product Information

• NCBI GI #: 308153472
• NCBI GeneID: 55023
• UniProt Accession #: Q8WWQ0; Q05CQ9; Q5VVH4; Q66I29; Q69YV1; Q8NBZ5; Q96H52; Q96ME2; Q9H261; A7J992; B2RPK4
• Molecular Weight: 1821
• NCBI Official Full Name: PH-interacting protein
• NCBI Official Synonym Full Names: pleckstrin homology domain interacting protein
• NCBI Official Symbol: PHIP
• NCBI Official Synonym Symbols: ndrp; BRWD2; WDR11; DCAF14
• NCBI Protein Information: PH-interacting protein; IRS-1 PH domain-binding protein; WD repeat-containing protein 11; DDB1 and CUL4 associated factor 14
• UniProt Protein Name: PH-interacting protein
• Protein Family: PH-interacting protein
• UniProt Gene Name: PHIP
• UniProt Synonym Gene Names: WDR11; PHIP
• UniProt Entry Name: PHIP_HUMAN

NCBI Description

PHIP binds the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS1; MIM 147545), modulates insulin signaling, and plays a role in pancreatic beta cell growth and survival (Farhang-Fallah et al., 2000 [PubMed 11018022]; Podcheko et al., 2007 [PubMed 17636024]).[supplied by OMIM, Jun 2009]

Uniprot Description

PHIP: Probable regulator of the insulin and insulin-like growth factor signaling pathways. Stimulates cell proliferation through regulation of cyclin transcription and has an anti- apoptotic activity through AKT1 phosphorylation and activation. Plays a role in the regulation of cell morphology and cytoskeletal organization. Interacts with IRS1 and IRS2. Interacts (via bromo domain) with acetylated lysine residues on histone H1.4, histone H3 and H4 (in vitro)

Chromosomal Location of Human Ortholog: 6q14

Cellular Component: nucleus

Molecular Function: protein binding; insulin receptor binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; regulation of cell shape; regulation of protein amino acid phosphorylation; positive regulation of mitosis; protein import into nucleus; regulation of growth; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; insulin receptor signaling pathway; regulation of cell morphogenesis; cytoskeleton organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; negative regulation of apoptosis