Mouse Ubiquitin carboxyl-terminal hydrolase 5 (USP5) ELISA Kit | USP5 elisa kit
Mouse Ubiquitin carboxyl-terminal hydrolase 5 (USP5) ELISA Kit
Gene Names
Usp5; ISOT; Ucht; ISOT-1; AA407472
Reactivity
Mouse
Synonyms
Ubiquitin carboxyl-terminal hydrolase 5 (USP5); Mouse Ubiquitin carboxyl-terminal hydrolase 5 (USP5) ELISA Kit; ISOT; ubiquitin isopeptidase T|ubiquitin specific peptidase 5|ubiquitin specific protease 5 (isopeptidase T)|ubiquitin-specific protease-5 (ubiquitin isopeptidase T); USP5 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse USP5. No significant cross-reactivity or interference between Mouse USP5 and analogues was observed.
Assay Type
Sandwich
Assay
Serum, Plasma, Other biological fluids
Analysis Method
Quantitive
Detection Wavelength
450nm
Sample Volume
1-200uL
Assay Duration
1-4.5h
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Test Principle
This assay employs a two-site sandwich ELISA to quantitate USP5 in samples. An antibody specific for USP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95,833 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 5
NCBI Official Synonym Full Names
ubiquitin specific peptidase 5 (isopeptidase T)
NCBI Official Symbol
Usp5
NCBI Official Synonym Symbols
ISOT; Ucht; ISOT-1; AA407472
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 5; isopeptidase T; ubiquitin thioesterase 5; deubiquitinating enzyme 5; ubiquitin thiolesterase 5; ubiquitin specific protease 5; ubiquitin carboxy terminal hydrolase T; ubiquitin-specific-processing protease 5
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 5
UniProt Gene Name
Usp5
UniProt Synonym Gene Names
Isot
UniProt Entry Name
UBP5_MOUSE
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Product Notes
The Mouse USP5 usp5 (Catalog #AAA2801976) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2801976 ELISA Kit recognizes Mouse USP5. It is sometimes possible for the material contained within the vial of "Ubiquitin carboxyl-terminal hydrolase 5 (USP5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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