Human Myelin and lymphocyte protein (MAL) ELISA Kit | MAL elisa kit

Human Myelin and lymphocyte protein (MAL) ELISA Kit

Cat. Num. AAA2801733

• Reactivity: Human
• Synonyms: Myelin and lymphocyte protein (MAL); Human Myelin and lymphocyte protein (MAL) ELISA Kit; T-cell differentiation protein MAL; T-lymphocyte maturation-associated protein; myelin and lymphocyte protein; MAL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MAL. No significant cross-reactivity or interference between Human MAL and analogues was observed.

• Asssay Type: Quantitive Sandwich
• Sample Types: Serum, Plasma, Other biological fluids
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV<8%; Inter-Assay: CV<12%
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate MAL in samples. An antibody specific for MAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAL bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Assay Duration: 1-4.5h
• Preparation and Storage: Store at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 4505091
• NCBI GeneID: 4118
• NCBI Accession #: NP_002362.1; NP_071884.1
• NCBI GenBank Nucleotide #: NM_002371.3
• UniProt Accession #: P21145; Q6FH77
• Molecular Weight: 5,989 Da
• NCBI Official Full Name: myelin and lymphocyte protein isoform a
• NCBI Official Synonym Full Names: mal, T-cell differentiation protein
• NCBI Official Symbol: MAL
• NCBI Protein Information: myelin and lymphocyte protein
• UniProt Protein Name: Myelin and lymphocyte protein
• Protein Family: Myelin and lymphocyte protein
• UniProt Gene Name: MAL
• UniProt Entry Name: MAL_HUMAN

NCBI Description

The protein encoded by this gene is a highly hydrophobic integral membrane protein belonging to the MAL family of proteolipids. The protein has been localized to the endoplasmic reticulum of T-cells and is a candidate linker protein in T-cell signal transduction. In addition, this proteolipid is localized in compact myelin of cells in the nervous system and has been implicated in myelin biogenesis and/or function. The protein plays a role in the formation, stabilization and maintenance of glycosphingolipid-enriched membrane microdomains. Down-regulation of this gene has been associated with a variety of human epithelial malignancies. Alternative splicing produces four transcript variants which vary from each other by the presence or absence of alternatively spliced exons 2 and 3. [provided by RefSeq, May 2012]