Rat Neurexin-1-alpha (NRXN1) ELISA Kit | NRXN1 elisa kit

Rat Neurexin-1-alpha (NRXN1) ELISA Kit

Cat. Num. AAA2801703

• Gene Names: Nrxn1; Nrxn1b
• Reactivity: Rat
• Synonyms: Neurexin-1-alpha (NRXN1); Rat Neurexin-1-alpha (NRXN1) ELISA Kit; DKFZp313P2036; FLJ35941; Hs.22998; KIAA0578; OTTHUMP00000200525; neurexin I; NRXN1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat NRXN1. No significant cross-reactivity or interference between Rat NRXN1 and analogues was observed.

• Asssay Type: Quantitive Sandwich
• Sample Types: Serum, Plasma, Other biological fluids
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV<8%; Inter-Assay: CV<12%
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate NRXN1 in samples. An antibody specific for NRXN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNRXN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRXN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRXN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Assay Duration: 1-4.5h
• Preparation and Storage: Store at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 37620151
• NCBI GeneID: 60391
• NCBI Accession #: NP_068535.2
• NCBI GenBank Nucleotide #: NM_021767.2
• UniProt Accession #: Q63372; Q63373
• Molecular Weight: 167,923 Da
• NCBI Official Full Name: neurexin 1
• NCBI Official Synonym Full Names: neurexin 1
• NCBI Official Symbol: Nrxn1
• NCBI Official Synonym Symbols: Nrxn1b
• NCBI Protein Information: neurexin 1; neurexin I-beta; neurexin-1-beta; neurexin I-alpha; neurexin-1-alpha; non-processed neurexin I-alpha
• UniProt Protein Name: Neurexin-1-alpha
• Protein Family: Neurexin
• UniProt Gene Name: Nrxn1
• UniProt Entry Name: NRX1A_RAT

NCBI Description

neuronal cell recognition molecule; may have a role in synaptogenesis [RGD]

Uniprot Description

NRXN1: Cell surface protein involved in cell-cell-interactions, excytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)- triggered exocytosis of secretory granules in pituitary gland. They may effect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latroxin from spider venom. Belongs to the neurexin family. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: presynaptic membrane; cell surface; endocytic vesicle; cell; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding; calcium channel regulator activity; acetylcholine receptor binding; calcium ion binding; cell adhesion molecule binding; calcium-dependent protein binding; receptor binding

Biological Process: prepulse inhibition; neurotransmitter secretion; vocal learning; social behavior; learning; positive regulation of synaptic transmission, glutamatergic; cerebellar granule cell differentiation; negative regulation of filopodium formation; heterophilic cell adhesion; positive regulation of synaptogenesis; synaptic transmission; establishment of protein localization; synaptogenesis; adult behavior; calcium-dependent cell-cell adhesion; protein heterotetramerization; neuron adhesion; angiogenesis; cell adhesion; neuromuscular process controlling balance; vesicle docking during exocytosis