Human Proprotein convertase subtilisin/kexin type 6 (PCSK6) ELISA Kit | PCSK6 elisa kit

Human Proprotein convertase subtilisin/kexin type 6 (PCSK6) ELISA Kit

Cat. Num. AAA2801689

• Gene Names: PCSK6; SPC4; PACE4
• Reactivity: Human
• Synonyms: Proprotein convertase subtilisin/kexin type 6 (PCSK6); Human Proprotein convertase subtilisin/kexin type 6 (PCSK6) ELISA Kit; PACE4; SPC4; paired basic amino acid cleaving system 4; subtilisin-like proprotein convertase 4; subtilisin-like protease; subtilisin/kexin-like protease PACE4; PCSK6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PCSK6. No significant cross-reactivity or interference between Human PCSK6 and analogues was observed.

• Asssay Type: Quantitive Sandwich
• Sample Types: Serum, Plasma, Other biological fluids
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV<8%; Inter-Assay: CV<12%
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate PCSK6 in samples. An antibody specific for PCSK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Assay Duration: 1-4.5h
• Preparation and Storage: Store at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 604723363
• NCBI GeneID: 5046
• NCBI Accession #: NP_001278238.1
• NCBI GenBank Nucleotide #: NM_001291309.1
• UniProt Accession #: P29122; Q15099; Q15100; Q9UEG7; Q9UEJ1; Q9UEJ2; Q9UEJ7; Q9UEJ8; Q9UEJ9; Q9Y4G9; Q9Y4H0; Q9Y4H1
• Molecular Weight: 105,375 Da
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 6 isoform i preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 6
• NCBI Official Symbol: PCSK6
• NCBI Official Synonym Symbols: SPC4; PACE4
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 6
• UniProt Protein Name: Proprotein convertase subtilisin/kexin type 6
• Protein Family: Proprotein convertase subtilisin/kexin
• UniProt Gene Name: PCSK6
• UniProt Synonym Gene Names: PACE4; SPC4
• UniProt Entry Name: PCSK6_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2014]

Uniprot Description

PCSK6: Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Protease; Secreted, signal peptide; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: cell surface; endoplasmic reticulum; extracellular matrix; extracellular space; Golgi lumen; membrane

Molecular Function: endopeptidase activity; heparin binding; nerve growth factor binding; serine-type endopeptidase activity

Biological Process: determination of left/right symmetry; glycoprotein metabolic process; nerve growth factor processing; nerve growth factor production; peptide hormone processing; protein processing; regulation of BMP signaling pathway; secretion by cell; zygotic determination of anterior/posterior axis, embryo