Human Visceral adipose-specific serine protease inhibitor (vaspin) ELISA Kit | vaspin elisa kit

Human Visceral adipose-specific serine protease inhibitor (vaspin) ELISA Kit

Cat. Num. AAA2801656

• Reactivity: Human
• Synonyms: Visceral adipose-specific serine protease inhibitor (vaspin); Human Visceral adipose-specific serine protease inhibitor (vaspin) ELISA Kit; OL-64; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 12; vaspin; visceral adipose-specific SERPIN; vaspin elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human SERPINA12. No significant cross-reactivity or interference between Human SERPINA12 and analogues was observed.

• Asssay Type: Quantitive Sandwich
• Sample Types: Serum, Plasma, Other biological fluids
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV<8%; Inter-Assay: CV<12%
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate SERPINA12 in samples. An antibody specific for SERPINA12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Assay Duration: 1-4.5h
• Preparation and Storage: Store at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 27777657
• NCBI Accession #: NP_776249.1
• NCBI GenBank Nucleotide #: NP_776249.1
• UniProt Accession #: Q8IW75
• NCBI Official Full Name: serpin A12
• UniProt Protein Name: Serpin A12
• UniProt Gene Name: SERPINA12
• UniProt Synonym Gene Names: Vaspin
• UniProt Entry Name: SPA12_HUMAN

Uniprot Description

SERPINA12: May modulate insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. Belongs to the serpin family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: extracellular space; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; negative regulation of lipid biosynthetic process; negative regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway