Human E3 ubiquitin-protein ligase MARCH1 (MARCH1) ELISA Kit | MARCH1 elisa kit

Human E3 ubiquitin-protein ligase MARCH1 (MARCH1) ELISA Kit

Cat. Num. AAA2800264

• Gene Names: MARCH1; RNF171; MARCH-I
• Reactivity: Human
• Synonyms: E3 ubiquitin-protein ligase MARCH1 (MARCH1); Human E3 ubiquitin-protein ligase MARCH1 (MARCH1) ELISA Kit; DKFZp564M1682; FLJ20668; MARCH-I; RNF171; membrane-associated RING-CH protein I; MARCH1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MARCH1. No significant cross-reactivity or interference between Human MARCH1 and analogues was observed.
• Sequence Length: 289

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MARCH1 elisa kit

This assay employs a two-site sandwich ELISA to quantitate MARCH1 in samples. An antibody specific for MARCH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MARCH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MARCH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MARCH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 261823980
• NCBI GeneID: 55016
• NCBI Accession #: NP_001159845.1
• NCBI GenBank Nucleotide #: NM_001166373.1
• UniProt Accession #: Q8TCQ1; Q9NWR0; D3DP29
• Molecular Weight: 30,873 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH1 isoform 1
• NCBI Official Synonym Full Names: membrane associated ring-CH-type finger 1
• NCBI Official Symbol: MARCH1
• NCBI Official Synonym Symbols: RNF171; MARCH-I
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH1
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH1
• UniProt Gene Name: MARCH1
• UniProt Synonym Gene Names: RNF171; MARCH-I

NCBI Description

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).[supplied by OMIM, Mar 2010]

Uniprot Description

E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.