Human DCN1-like protein 5 (DCUN1D5) ELISA Kit | DCUN1D5 elisa kit
Human DCN1-like protein 5 (DCUN1D5) ELISA Kit
Gene Names
DCUN1D5; SCCRO5
Reactivity
Human
Synonyms
DCN1-like protein 5 (DCUN1D5); Human DCN1-like protein 5 (DCUN1D5) ELISA Kit; FLJ32431; FLJ37425; MGC2714; DCN1; defective in cullin neddylation 1; domain containing 5; DCUN1D5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human DCUN1D5. No significant cross-reactivity or interference between Human DCUN1D5 and analogues was observed.
Sequence Length
237
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other Biological Fluids
Detection Wavelength
450 nm
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%)
= SD/meanX100
Intra-Assay
CV
Inter-Assay
CV
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for DCUN1D5 elisa kit
Description: This assay employs a two-site sandwich ELISA to quantitate DCUN1D5 in samples. An antibody specific for DCUN1D5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DCUN1D5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCUN1D5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCUN1D5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: DCUN1D5 Contains 1 DCUN1 domain.SCF-type E3 ubiquitin ligases are multi-protein complexes required for polyubiquitination and subsequent degradation of target proteins by the 26S proteasome.C. elegans DCN-1 and S. cerevisiae Dcn1p (defective in cullin neddylation) are characterized by a novel UBA-like ubiquitin-binding domain and a DUF298 domain of unknown function. Consistent with their requirements for neddylation, DCN-1 and Dcn1p directly bind Nedd8 and physically associate with cullins in both species. Moreover, overexpression of Dcn1p in yeast results in the accumulation of Nedd8-modified cullin Cdc53p. Both in vivo and in vitro experiments indicate that Dcn1p does not inhibit deneddylation of Cdc53p by the COP9 signalosome, but greatly increases the kinetics of the neddylation reaction.
Overview: DCUN1D5 Contains 1 DCUN1 domain.SCF-type E3 ubiquitin ligases are multi-protein complexes required for polyubiquitination and subsequent degradation of target proteins by the 26S proteasome.C. elegans DCN-1 and S. cerevisiae Dcn1p (defective in cullin neddylation) are characterized by a novel UBA-like ubiquitin-binding domain and a DUF298 domain of unknown function. Consistent with their requirements for neddylation, DCN-1 and Dcn1p directly bind Nedd8 and physically associate with cullins in both species. Moreover, overexpression of Dcn1p in yeast results in the accumulation of Nedd8-modified cullin Cdc53p. Both in vivo and in vitro experiments indicate that Dcn1p does not inhibit deneddylation of Cdc53p by the COP9 signalosome, but greatly increases the kinetics of the neddylation reaction.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,508 Da
NCBI Official Full Name
DCN1-like protein 5 isoform a
NCBI Official Synonym Full Names
defective in cullin neddylation 1 domain containing 5
NCBI Official Symbol
DCUN1D5
NCBI Official Synonym Symbols
SCCRO5
NCBI Protein Information
DCN1-like protein 5
UniProt Protein Name
DCN1-like protein 5
Protein Family
UniProt Gene Name
DCUN1D5
Research Articles on DCUN1D5
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Product Notes
The Human DCUN1D5 dcun1d5 (Catalog #AAA2800160) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2800160 ELISA Kit recognizes Human DCUN1D5. It is sometimes possible for the material contained within the vial of "DCN1-like protein 5 (DCUN1D5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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