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WB (Western Blot)
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WB (Western Blot) (Western blot analysis of extracts from serum-starved Hela treated with TPA (100 nM, 15 min), using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:10000, 1:100000, 1:500000 dilution.(Validation Experiment))

CREB Recombinant Antibody | CREB1 recombinant antibody

Phospho-CREB (Ser133) Antibody, Rabbit MAb

Reactivity
Human
Applications
Functional Assay, Western Blot, Functional Assay, Immunohistochemistry
Purity
Protein A
Synonyms
CREB; Monoclonal Recombinant Antibody; Phospho-CREB (Ser133) Antibody; Rabbit MAb; cAMP responsive element binding protein 1; Anti-CREB Antibody; Anti-CREB-1 Antibody; CREB1 recombinant antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
0049
Specificity
Reacts with: Human Phospho-CREB (Ser133), may cross with Phospho-ATF-1 (Ser63) by sequence similarity. Predicted to React with: Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovine, Pig, Goat
Purity/Purification
Protein A
Form/Format
Liquid; 10 mM sodium HEPES, 150 mM NaCl, 100ug/mL BSA, 50% glycerol, pH 7.5
Applicable Applications for CREB1 recombinant antibody
Western Blot (WB)(FAQ Protocol), Immunohistochemistry-Paraffin (IHC-P)(FAQ Protocol)
Application Notes
WB: 1:10000-1:200000
IHC-P: 1:100-1:500
Immunogen
A synthetic phosphopeptide corresponding to residues around Ser133 of human CREB.
Preparation
This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser133 of human CREB.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

WB (Western Blot)

(Western blot analysis of extracts from serum-starved Hela treated with TPA (100 nM, 15 min), using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:10000, 1:100000, 1:500000 dilution.(Validation Experiment))

WB (Western Blot) (Western blot analysis of extracts from serum-starved Hela treated with TPA (100 nM, 15 min), using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:10000, 1:100000, 1:500000 dilution.(Validation Experiment))

WB (Western Blot)

(Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TPA (100 nM, 15 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:200000 dilution.(Validation Experiment))

WB (Western Blot) (Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TPA (100 nM, 15 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:200000 dilution.(Validation Experiment))

WB (Western Blot)

(Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TPA (100 nM, 15 min)(line B); treated with TPA and lambda-phosphatase (line C) using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:200000 dilution.(Validation Experiment))

WB (Western Blot) (Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TPA (100 nM, 15 min)(line B); treated with TPA and lambda-phosphatase (line C) using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:200000 dilution.(Validation Experiment))

IHC (Immunohistochemistry-Paraffin)

(Immunohistochemical analysis of paraffin-embedded human renal carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) Antibody, Rabbit Monoclonal (#AAA27785) at 1:200 dilution.)

IHC (Immunohistochemistry-Paraffin) (Immunohistochemical analysis of paraffin-embedded human renal carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) Antibody, Rabbit Monoclonal (#AAA27785) at 1:200 dilution.)

IHC (Immunohistochemistry-Paraffin)

(Immunohistochemical analysis of paraffin-embedded human lung cancer, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) Antibody, Rabbit Monoclonal (#AAA27785) at 1:200 dilution.)

IHC (Immunohistochemistry-Paraffin) (Immunohistochemical analysis of paraffin-embedded human lung cancer, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) Antibody, Rabbit Monoclonal (#AAA27785) at 1:200 dilution.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human breast cancer, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) Antibody, Rabbit Monoclonal (#AAA27785) at 1:200 dilution.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) Antibody, Rabbit Monoclonal (#AAA27785) at 1:200 dilution.)

WB (Western Blot)

(Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with TPA (200 nM, 15 min; +), using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:200000 dilution (upper) or Anti-CREB Antibody, Rabbit Polyclonal at 1:500 dilution (middle) or Beta-Tubulin Loading Control Antibody, Rabbit MAb (Chimeric) at 1:40000 dilution (lower). Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) may cross with Phospho-ATF-1 (Ser63) by sequence similarity.)

WB (Western Blot) (Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with TPA (200 nM, 15 min; +), using Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) at 1:200000 dilution (upper) or Anti-CREB Antibody, Rabbit Polyclonal at 1:500 dilution (middle) or Beta-Tubulin Loading Control Antibody, Rabbit MAb (Chimeric) at 1:40000 dilution (lower). Phospho-CREB (Ser133) rabbit monoclonal Antibody (#AAA27785) may cross with Phospho-ATF-1 (Ser63) by sequence similarity.)
Related Product Information for CREB1 recombinant antibody
CAMP responsive element binding protein 1, also known as CREB-1, plays multiple functions as a transcription factor in gene regulation. This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. CREB1 proteins are also known to be expressed in several spliced isoforms that act as transcriptional activators or repressors. The activator isoforms, possessing the functional domains for kinase induction and for interaction with other transcriptional regulators, act as transcriptional activators. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB-1 has a complex influence on behavioural responses to drugs of abuse which varies depending on the brain region in which it is expressed. CREB-1 is important for serotonin (5-HT)-induced long-term facilitation (LTF) of the sensorimotor synapse in Aplysia.
Product Categories/Family for CREB1 recombinant antibody
References
Sadamot. H. e. al. (2011. Direc. observatio. o. dimerizatio. betwee. differen. CREB. isoform. i. . livin. cell. PLo. One. . (6). e20285.Li. RY. e. al. (2011. Th. requiremen. fo. enhance. CREB. expressio. i. consolidatio. o. long-ter. synapti. facilitatio. an. long-ter. excitabilit. i. sensor. neuron. o. Aplysia. . Neurosci. 3. (18). 6871-9.Hoeffle. JP. e. al. (1988. Cycli. AMP-responsiv. DNA-bindin. protein. structur. base. o. . clone. placenta. cDNA. Science. 24. (4884). 1430-3.

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Product Notes

The CREB1 (Catalog #AAA27785) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CREB (Ser133) Antibody, Rabbit MAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CREB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB)(FAQ Protocol), Immunohistochemistry-Paraffin (IHC-P)(FAQ Protocol). WB: 1:10000-1:200000 IHC-P: 1:100-1:500. Researchers should empirically determine the suitability of the CREB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CREB, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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