Human Multidrug Resistance Associated Protein 1 (MRP1) ELISA Kit | MRP1 elisa kit

Human Multidrug Resistance Associated Protein 1 (MRP1)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2709641

• Reactivity: Human
• Applications: ELISA
• Synonyms: Multidrug Resistance Associated Protein 1 (MRP1); Human Multidrug Resistance Associated Protein 1 (MRP1)(Sandwich ELISA) ELISA Kit; ELISA Kit for Multidrug Resistance Associated Protein 1 (MRP1); MRP1/Multidrug Resistance Associated Protein 1; ABCC1; ABC29; ABCC; GS-X; ATP-Binding Cassette; Subfamily C(CFTR/MRP)Member 1; Leukotriene C(4) transporter; MRP1 elisa kit

• Reactivity: Human
• Specificity: Reactive with Human Multidrug Resistance Associated Protein 1 (MRP1); This assay has high sensitivity and excellent specificity for detection of Multidrug Resistance Associated Protein 1 (MRP1). No significant cross-reactivity or interference between Multidrug Resistance Associated Protein 1 (MRP1) and analogues was observed.

• Applicable Applications for MRP1 elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Detection Method: Double-antibody Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Assay Length: 3 h
• Sample Size: 100ul (Please refer to the manual for the specific data.)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Multidrug Resistance Associated Protein 1 (MRP1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Multidrug Resistance Associated Protein 1 (MRP1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transferrin Receptor 2 (TFR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transferrin Receptor 2 (TFR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transferrin Receptor 2 (TFR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transferrin Receptor 2 (TFR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for MRP1 elisa kit

Tumor immunity