Human Wingless Type MMTV Integration Site Family, Member 2 (WNT2) CLIA Kit | WNT2 clia kit

Human Wingless Type MMTV Integration Site Family, Member 2 (WNT2) CLIA Kit

Cat. Num. AAA2709227

• Gene Names: WNT2; IRP; INT1L1
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Wingless Type MMTV Integration Site Family; Member 2 (WNT2); Human Wingless Type MMTV Integration Site Family; Member 2 (WNT2) CLIA Kit; Member 2; IRP; INT1L1; Secreted Growth Factor; Int-1-related protein; WNT2 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 2 (WNT2).; No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 2 (WNT2) and analogues was observed.
• Sequence Length: 360

• Applicable Applications for WNT2 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.2pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 2 (WNT2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 2 (WNT2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 2 (WNT2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 2 (WNT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 2 (WNT2) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 4507927
• NCBI GeneID: 7472
• NCBI Accession #: NP_003382.1
• NCBI GenBank Nucleotide #: NP_003382.1
• UniProt Accession #: P09544
• NCBI Official Full Name: protein Wnt-2
• NCBI Official Synonym Full Names: Wnt family member 2
• NCBI Official Symbol: WNT2
• NCBI Official Synonym Symbols: IRP; INT1L1
• NCBI Protein Information: protein Wnt-2
• UniProt Protein Name: Protein Wnt-2
• UniProt Gene Name: WNT2
• UniProt Synonym Gene Names: INT1L1; IRP; IRP
• UniProt Entry Name: WNT2_HUMAN

NCBI Description

This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT2: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted; Cell development/differentiation; Cell adhesion

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: cytoplasm; extracellular matrix; extracellular region; extracellular space; plasma membrane; proteinaceous extracellular matrix

Molecular Function: cytokine activity; frizzled binding; protein binding; receptor agonist activity

Biological Process: atrial cardiac muscle morphogenesis; cell fate commitment; cell proliferation in midbrain; cell-cell signaling; lens development in camera-type eye; lung development; neurogenesis; neuron differentiation; positive regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of neurogenesis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin