Rat Platelet Derived Growth Factor Subunit B (PDGFB) CLIA Kit | PDGFB clia kit

Rat Platelet Derived Growth Factor Subunit B (PDGFB) CLIA Kit

Cat. Num. AAA2709135

• Gene Names: Pdgfb; SIS; c-sis
• Reactivity: Rat
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Platelet Derived Growth Factor Subunit B (PDGFB); Rat Platelet Derived Growth Factor Subunit B (PDGFB) CLIA Kit; Platelet Derived Growth Factor Subunit B; PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral(v-sis)Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin; PDGFB clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit B (PDGFB).; No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit B (PDGFB) and analogues was observed.
• Sequence Length: 241

• Applicable Applications for PDGFB clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 2.74-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 1.24pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Derived Growth Factor Subunit B (PDGFB) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 158081747
• NCBI GeneID: 24628
• NCBI Accession #: NP_113712.1
• NCBI GenBank Nucleotide #: NP_113712.1
• UniProt Accession #: Q05028
• NCBI Official Full Name: platelet-derived growth factor subunit B
• NCBI Official Synonym Full Names: platelet derived growth factor subunit B
• NCBI Official Symbol: Pdgfb
• NCBI Official Synonym Symbols: SIS; c-sis
• NCBI Protein Information: platelet-derived growth factor subunit B
• UniProt Protein Name: Platelet-derived growth factor subunit B
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: Pdgfb
• UniProt Synonym Gene Names: PDGF subunit B
• UniProt Entry Name: PDGFB_RAT

NCBI Description

may play a role in carotid artery smooth muscle cell proliferation and neointimal formation in response to balloon catheter injury [RGD, Feb 2006]

Uniprot Description

PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Oncoprotein

Cellular Component: extracellular space; cell surface; membrane; basolateral plasma membrane; cytoplasm

Molecular Function: collagen binding; identical protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; superoxide-generating NADPH oxidase activator activity; chemoattractant activity; receptor binding

Biological Process: positive regulation of cyclin-dependent protein kinase activity; heart development; positive regulation of transcription, DNA-dependent; positive regulation of fibroblast growth factor receptor signaling pathway; cell projection biogenesis; response to organic cyclic substance; protein amino acid phosphorylation; monocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; positive chemotaxis; cell growth; positive regulation of mitotic cell cycle, embryonic; substrate-bound cell migration; response to drug; positive regulation of mitosis; positive regulation of chemotaxis; activation of protein kinase B; positive regulation of blood vessel endothelial cell migration; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; response to estrogen stimulus; activation of protein kinase activity; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; negative regulation of transcription, DNA-dependent; actin cytoskeleton organization and biogenesis; embryonic placenta development; peptidyl-tyrosine phosphorylation; wound healing; positive regulation of smooth muscle cell proliferation; platelet-derived growth factor receptor signaling pathway; positive regulation of smooth muscle cell migration; response to estradiol stimulus; response to insulin stimulus; response to organic substance; positive regulation of MAPKKK cascade; positive regulation of glomerular filtration; positive regulation of cell proliferation; response to wounding; hemopoiesis; DNA replication; regulation of peptidyl-tyrosine phosphorylation; negative regulation of cell migration; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of phosphoinositide 3-kinase activity; regulation of cell proliferation; peptidyl-serine phosphorylation; response to hypoxia; blood coagulation; positive regulation of DNA replication; positive regulation of cell migration