Human Collagen Type X (COL10) CLIA Kit | COL10 clia kit

Human Collagen Type X (COL10) CLIA Kit

Cat. Num. AAA2709074

• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Collagen Type X (COL10); Human Collagen Type X (COL10) CLIA Kit; Collagen Type X; COL10A1; COL10-A1; Schmid Metaphyseal Chondrodysplasia; Collagen Alpha-1(X)chain; COL10 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type X (COL10).; No significant cross-reactivity or interference between Collagen Type X (COL10) and analogues was observed.
• Sequence Length: 680

• Applicable Applications for COL10 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.57pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type X (COL10) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type X (COL10) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Collagen Type X (COL10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Collagen Type X (COL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Collagen Type X (COL10) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 119568625
• NCBI GeneID: 1300
• UniProt Accession #: Q03692
• NCBI Official Full Name: collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia)
• NCBI Official Synonym Full Names: collagen type X alpha 1 chain
• NCBI Official Symbol: COL10A1
• NCBI Protein Information: collagen alpha-1(X) chain
• UniProt Protein Name: Collagen alpha-1(X) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL10A1
• UniProt Entry Name: COAA1_HUMAN

NCBI Description

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]

Uniprot Description

COL10A1: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage. Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD). SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q21-q22

Cellular Component: proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; extracellular region; cell cortex

Molecular Function: metal ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; cartilage development; skeletal development; endochondral ossification

Disease: Metaphyseal Chondrodysplasia, Schmid Type