Typical Testing Data/Standard Curve (for reference only)
Human Serpin B3 (SERPINB3) CLIA Kit | SERPINB3 clia kit
Human Serpin B3 (SERPINB3) CLIA Kit
Gene Names
SERPINB3; SCC; T4-A; SCCA1; SSCA1; SCCA-1; HsT1196; SCCA-PD
Reactivity
Human
Applications
Chemiluminescence Immunoassay (CLIA)
Synonyms
Serpin B3 (SERPINB3); Human Serpin B3 (SERPINB3) CLIA Kit; Serpin B3; SCCA1; T4-A; Serpin Peptidase Inhibitor; Clade B(ovalbumin); Member 3; SERPINB3 clia kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Serpin B3 (SERPINB3).
No significant cross-reactivity or interference between Serpin B3 (SERPINB3) and analogues was observed.
No significant cross-reactivity or interference between Serpin B3 (SERPINB3) and analogues was observed.
Sequence Length
390
Applicable Applications for SERPINB3 clia kit
Chemiluminescence Immunoassay (CLIA)
Assay Type
Double-antibody Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
13.7-10,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 5.4pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serpin B3 (SERPINB3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serpin B3 (SERPINB3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serpin B3 (SERPINB3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Serpin B3 (SERPINB3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Serpin B3 (SERPINB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Serpin B3 (SERPINB3) level in the sample or standard.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serpin B3
NCBI Official Synonym Full Names
serpin family B member 3
NCBI Official Symbol
SERPINB3
NCBI Official Synonym Symbols
SCC; T4-A; SCCA1; SSCA1; SCCA-1; HsT1196; SCCA-PD
NCBI Protein Information
serpin B3
UniProt Protein Name
Serpin B3
Protein Family
UniProt Gene Name
SERPINB3
UniProt Synonym Gene Names
SCCA; SCCA1; SCCA-1
UniProt Entry Name
SPB3_HUMAN
Uniprot Description
SERPINB3: May act as a protease inhibitor to modulate the host immune response against tumor cells. Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 18q21.3
Cellular Component: cytoplasm; cytoplasmic vesicle; extracellular space; nucleus; vesicle
Molecular Function: cysteine protease inhibitor activity; protease binding; serine-type endopeptidase inhibitor activity; viral receptor activity
Biological Process: negative regulation of catalytic activity; negative regulation of JNK activity; negative regulation of peptidase activity; negative regulation of proteolysis; positive regulation of cell migration; positive regulation of cell proliferation
Research Articles on SERPINB3
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Product Notes
The Human SERPINB3 serpinb3 (Catalog #AAA2708924) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2708924 CLIA Kit recognizes Human SERPINB3. AAA Biotech's Serpin B3 (SERPINB3) can be used in a range of immunoassay formats including, but not limited to, Chemiluminescence Immunoassay (CLIA). Researchers should empirically determine the suitability of the SERPINB3 serpinb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serpin B3 (SERPINB3), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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