Mouse Interleukin 33 (IL33) ELISA Kit | IL33 elisa kit

Mouse Interleukin 33 (IL33) Mini Samples ELISA Kit

Cat. Num. AAA2708375

• Gene Names: Il33; Il-33; Il1f11; NF-HEV; 9230117N10Rik
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Interleukin 33 (IL33); Mouse Interleukin 33 (IL33) Mini Samples ELISA Kit; Interleukin 33; DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family; Member 11; Nuclear factor from high endothelial venules; IL33 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 33 (IL33).; No significant cross-reactivity or interference between Mini Samples Interleukin 33 (IL33) and analogues was observed.
• Sequence Length: 266

• Applicable Applications for IL33 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 33 (IL33) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 33 (IL33) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 33 (IL33). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 33 (IL33). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 33 (IL33), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Interleukin 33 (IL33) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 257900495
• NCBI GeneID: 77125
• NCBI Accession #: NP_001158196.1
• NCBI GenBank Nucleotide #: NP_001158196.1
• UniProt Accession #: Q8BVZ5
• NCBI Official Full Name: interleukin-33
• NCBI Official Synonym Full Names: interleukin 33
• NCBI Official Symbol: Il33
• NCBI Official Synonym Symbols: Il-33; Il1f11; NF-HEV; 9230117N10Rik
• NCBI Protein Information: interleukin-33
• UniProt Protein Name: Interleukin-33
• Protein Family: Interleukin
• UniProt Gene Name: Il33
• UniProt Synonym Gene Names: IL-33
• UniProt Entry Name: IL33_MOUSE

Uniprot Description

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Cellular Component: nucleus

Molecular Function: cytokine activity

Biological Process: defense response to virus; macrophage activation during immune response; microglial cell activation during immune response; negative regulation of immunoglobulin secretion; negative regulation of interferon-gamma production; negative regulation of leukocyte migration; negative regulation of T-helper 1 type immune response; positive regulation of immunoglobulin secretion; positive regulation of inflammatory response; positive regulation of interleukin-13 production; positive regulation of interleukin-4 production; positive regulation of interleukin-5 production; positive regulation of interleukin-6 production; positive regulation of macrophage activation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of T-helper 2 type immune response; positive regulation of transcription from RNA polymerase II promoter