Human Follicle Stimulating Hormone (FSH) ELISA Kit | FSH elisa kit

Human Follicle Stimulating Hormone (FSH) Instant ELISA Kit

Cat. Num. AAA2708181

• Gene Names: CGA; HCG; LHA; FSHA; GPA1; GPHa; TSHA; GPHA1; CG-ALPHA
• Reactivity: Human
• Applications: ELISA
• Synonyms: Follicle Stimulating Hormone (FSH); Human Follicle Stimulating Hormone (FSH) Instant ELISA Kit; Follicle Stimulating Hormone; FSH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Instant Follicle Stimulating Hormone (FSH).; No significant cross-reactivity or interference between Instant Follicle Stimulating Hormone (FSH) and analogues was observed.
• Sequence Length: 116

• Applicable Applications for FSH elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.78-50mIU/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.31mIU/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Follicle Stimulating Hormone (FSH). Standards or samples and HRP-labeled detection antibody specific to Instant Follicle Stimulating Hormone (FSH) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Follicle Stimulating Hormone (FSH), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Instant Follicle Stimulating Hormone (FSH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 546845
• NCBI GeneID: 1081
• UniProt Accession #: P01215
• NCBI Official Full Name: thyroid-stimulating hormone alpha subunit
• NCBI Official Synonym Full Names: glycoprotein hormones, alpha polypeptide
• NCBI Official Symbol: CGA
• NCBI Official Synonym Symbols: HCG; LHA; FSHA; GPA1; GPHa; TSHA; GPHA1; CG-ALPHA
• NCBI Protein Information: glycoprotein hormones alpha chain
• UniProt Protein Name: Glycoprotein hormones alpha chain
• UniProt Gene Name: CGA
• UniProt Synonym Gene Names: CG-alpha; FSH-alpha; LSH-alpha; TSH-alpha
• UniProt Entry Name: GLHA_HUMAN

NCBI Description

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CGA: The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q12-q21

Cellular Component: extracellular region

Molecular Function: protein binding; hormone activity

Biological Process: cellular protein metabolic process; cell-cell signaling; peptide hormone processing; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of cell migration