Human Bradykinin (BK) ELISA Kit | BK elisa kit

Human Bradykinin (BK) High Sensitive ELISA Kit

Cat. Num. AAA2708041

• Gene Names: PNLIP; PL; PTL; PNLIPD
• Reactivity: Human
• Applications: ELISA
• Synonyms: Bradykinin (BK); Human Bradykinin (BK) High Sensitive ELISA Kit; Bradykinin; BK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Bradykinin (BK).; No significant cross-reactivity or interference between High Sensitive Bradykinin (BK) and analogues was observed.
• Sequence Length: 465

• Applicable Applications for BK elisa kit: ELISA (EIA)
• Assay Type: Competitive Inhibition
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 1.23-100pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.65pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Bradykinin (BK) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Bradykinin (BK) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Bradykinin (BK) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Bradykinin (BK) and unlabeled High Sensitive Bradykinin (BK) (Standards or samples) with the pre-coated antibody specific to High Sensitive Bradykinin (BK). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Bradykinin (BK) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Bradykinin (BK) in the sample.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 1304379
• NCBI GeneID: 5406
• UniProt Accession #: P16233
• NCBI Official Full Name: pancreatic lipase
• NCBI Official Synonym Full Names: pancreatic lipase
• NCBI Official Symbol: PNLIP
• NCBI Official Synonym Symbols: PL; PTL; PNLIPD
• NCBI Protein Information: pancreatic triacylglycerol lipase
• UniProt Protein Name: Pancreatic triacylglycerol lipase
• UniProt Gene Name: PNLIP
• UniProt Synonym Gene Names: PL; PTL
• UniProt Entry Name: LIPP_HUMAN

NCBI Description

This gene encodes a member of the lipase family of proteins. The encoded enzyme is secreted by the pancreas and hydrolyzes triglycerides in the small intestine, and is essential for the efficient digestion of dietary fats. Inhibition of the encoded enzyme may prevent high-fat diet-induced obesity in mice and result in weight loss in human patients with obesity. Mutations in this gene cause congenital pancreatic lipase deficiency, a rare disorder characterized by steatorrhea. [provided by RefSeq, Jul 2016]

Uniprot Description

PNLIP: a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]

Protein type: EC 3.1.1.3; Lipid Metabolism - glycerolipid; Secreted; Secreted, signal peptide; Hydrolase

Chromosomal Location of Human Ortholog: 10q26.1

Cellular Component: extracellular region

Molecular Function: triacylglycerol lipase activity; metal ion binding

Biological Process: phototransduction, visible light; cholesterol absorption; retinoid metabolic process; lipid catabolic process; lipid digestion

Disease: Pancreatic Lipase Deficiency