Human Activated Protein C (APC) ELISA Kit | APC elisa kit

Human Activated Protein C (APC) High Sensitive ELISA Kit

Cat. Num. AAA2708027

• Gene Names: APC; GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46
• Reactivity: Human
• Applications: ELISA
• Synonyms: Activated Protein C (APC); Human Activated Protein C (APC) High Sensitive ELISA Kit; Activated Protein C; APC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Activated Protein C (APC).; No significant cross-reactivity or interference between High Sensitive Activated Protein C (APC) and analogues was observed.
• Sequence Length: 2843

• Applicable Applications for APC elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.6pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Activated Protein C (APC) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Activated Protein C (APC) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Activated Protein C (APC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Activated Protein C (APC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Activated Protein C (APC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Activated Protein C (APC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 53759122
• NCBI GeneID: 324
• NCBI Accession #: NP_000029.2
• NCBI GenBank Nucleotide #: NP_000029.2
• UniProt Accession #: P25054
• NCBI Official Full Name: adenomatous polyposis coli protein isoform b
• NCBI Official Synonym Full Names: APC regulator of WNT signaling pathway
• NCBI Official Symbol: APC
• NCBI Official Synonym Symbols: GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46
• NCBI Protein Information: adenomatous polyposis coli protein
• UniProt Protein Name: Adenomatous polyposis coli protein
• Protein Family: Adenomatous polyposis coli
• UniProt Gene Name: APC
• UniProt Synonym Gene Names: DP2.5; Protein APC
• UniProt Entry Name: APC_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Mutations in the APC gene have been found to occur in most colorectal cancers. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Dec 2019]

Uniprot Description

APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: centrosome; tight junction; cytosol; beta-catenin destruction complex; nucleoplasm; kinetochore; cell-cell adherens junction; lamellipodium; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus; lateral plasma membrane

Molecular Function: microtubule plus-end binding; protein binding; cadherin binding; gamma-catenin binding; microtubule binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: positive regulation of cell adhesion; somatic stem cell maintenance; apoptosis; positive regulation of apoptosis; positive regulation of microtubule polymerization; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; cytoplasmic microtubule organization and biogenesis; muscle maintenance; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of osteoclast differentiation; hair follicle development; cytokinesis after mitosis; mitotic cell cycle spindle assembly checkpoint; positive regulation of epithelial cell differentiation; protein complex assembly; cell adhesion; cell cycle arrest; kidney development; proximal/distal pattern formation; cell structure disassembly during apoptosis; regulation of microtubule-based process; regulation of attachment of spindle microtubules to kinetochore; negative regulation of odontogenesis; cell migration; thymus development; regulation of osteoblast differentiation; negative regulation of MAPKKK cascade; negative regulation of microtubule depolymerization; axis specification; negative regulation of cyclin-dependent protein kinase activity; positive regulation of pseudopodium formation; axonogenesis; dorsal/ventral pattern formation; retina development in camera-type eye; positive regulation of cell division; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; response to DNA damage stimulus; mitotic metaphase/anaphase transition; positive regulation of cell migration

Disease: Familial Adenomatous Polyposis 1; Gastric Cancer; Desmoid Disease, Hereditary; Colorectal Cancer; Hepatocellular Carcinoma