Human Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit | TFPI elisa kit

Human Tissue Factor Pathway Inhibitor (TFPI) High Sensitive ELISA Kit

Cat. Num. AAA2708000

• Gene Names: TFPI; EPI; TFI; LACI; TFPI1
• Reactivity: Human
• Applications: ELISA
• Synonyms: Tissue Factor Pathway Inhibitor (TFPI); Human Tissue Factor Pathway Inhibitor (TFPI) High Sensitive ELISA Kit; Tissue Factor Pathway Inhibitor; TFI; EPI; LACI; TFPI1; Lipoprotein-Associated Coagulation Inhibitor; Extrinsic pathway inhibitor; TFPI elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Tissue Factor Pathway Inhibitor (TFPI).; No significant cross-reactivity or interference between High Sensitive Tissue Factor Pathway Inhibitor (TFPI) and analogues was observed.
• Sequence Length: 251

• Applicable Applications for TFPI elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: plasma, urine, tissue homogenates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.057ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Tissue Factor Pathway Inhibitor (TFPI) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Tissue Factor Pathway Inhibitor (TFPI) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Tissue Factor Pathway Inhibitor (TFPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Tissue Factor Pathway Inhibitor (TFPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Tissue Factor Pathway Inhibitor (TFPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Tissue Factor Pathway Inhibitor (TFPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 73760409
• NCBI GeneID: 7035
• NCBI Accession #: NP_001027452.1
• NCBI GenBank Nucleotide #: NP_001027452.1
• UniProt Accession #: P10646
• NCBI Official Full Name: tissue factor pathway inhibitor isoform b
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor
• NCBI Official Symbol: TFPI
• NCBI Official Synonym Symbols: EPI; TFI; LACI; TFPI1
• NCBI Protein Information: tissue factor pathway inhibitor
• UniProt Protein Name: Tissue factor pathway inhibitor
• Protein Family: Tissue factor pathway inhibitor
• UniProt Gene Name: TFPI
• UniProt Synonym Gene Names: LACI; TFPI1; TFPI; EPI; LACI
• UniProt Entry Name: TFPI1_HUMAN

NCBI Description

This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. [provided by RefSeq, Jul 2016]

Uniprot Description

TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Membrane protein, GPI anchor; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: extracellular space; cell surface; endoplasmic reticulum; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: blood coagulation, extrinsic pathway; blood coagulation