Human Preprovasopressin (VP) ELISA Kit | VP elisa kit

Human Preprovasopressin (VP) ELISA Kit

Cat. Num. AAA2707646

• Gene Names: AVP; VP; ADH; ARVP; AVRP; AVP-NPII
• Reactivity: Human
• Synonyms: Preprovasopressin (VP); Human Preprovasopressin (VP) ELISA Kit; AVP-NPII; ARVP; AVP; Vasopressin-Neurophysin 2-Copeptin; Arginine-vasopressin; Neurophysin 2; Copeptin; VP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Preprovasopressin (VP).; No significant cross-reactivity or interference between Preprovasopressin (VP) and analogues was observed.
• Sequence Length: 164

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.6pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Preprovasopressin (VP) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Preprovasopressin (VP) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Preprovasopressin (VP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Preprovasopressin (VP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Preprovasopressin (VP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Preprovasopressin (VP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13259533
• NCBI GeneID: 551
• NCBI Accession #: NP_000481.2
• NCBI GenBank Nucleotide #: NP_000481.2
• UniProt Accession #: P01185
• NCBI Official Full Name: vasopressin-neurophysin 2-copeptin preproprotein
• NCBI Official Synonym Full Names: arginine vasopressin
• NCBI Official Symbol: AVP
• NCBI Official Synonym Symbols: VP; ADH; ARVP; AVRP; AVP-NPII
• NCBI Protein Information: vasopressin-neurophysin 2-copeptin
• UniProt Protein Name: Vasopressin-neurophysin 2-copeptin
• UniProt Gene Name: AVP
• UniProt Synonym Gene Names: ARVP; VP
• UniProt Entry Name: NEU2_HUMAN

NCBI Description

This gene encodes a member of the vasopressin/oxytocin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the neuropeptide hormone arginine vasopressin, and two other peptides, neurophysin 2 and copeptin. Arginine vasopressin is a posterior pituitary hormone that is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). This gene is present in a gene cluster with the related gene oxytocin on chromosome 20. [provided by RefSeq, Nov 2015]

Uniprot Description

AVP: Neurophysin 2 specifically binds vasopressin. Defects in AVP are the cause of diabetes insipidus, neurohypophyseal (NDI). A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopression deficiency during childhood. Belongs to the vasopressin/oxytocin family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular space; dendrite; extracellular region; cytosol; secretory granule

Molecular Function: neuropeptide hormone activity; signal transducer activity; V1A vasopressin receptor binding; caspase inhibitor activity; neurohypophyseal hormone activity; V1B vasopressin receptor binding; receptor binding; protein kinase activity

Biological Process: response to nicotine; circadian rhythm; water transport; positive regulation of glutamate secretion; positive regulation of cellular pH reduction; locomotory behavior; positive regulation of systemic arterial blood pressure; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; elevation of cytosolic calcium ion concentration; cell-cell signaling; hyperosmotic salinity response; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; transmembrane transport; positive regulation of prostaglandin biosynthetic process; penile erection; grooming behavior; vasoconstriction; sodium-independent organic anion transport; generation of precursor metabolites and energy; maternal behavior; response to testosterone stimulus; social behavior; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; negative regulation of female receptivity; response to ethanol; negative regulation of transmission of nerve impulse; positive regulation of vasoconstriction; renal water homeostasis; negative regulation of apoptosis

Disease: Diabetes Insipidus, Neurohypophyseal