Human Testican 1 (TIC1) ELISA Kit | TIC1 elisa kit

Human Testican 1 (TIC1) ELISA Kit

Cat. Num. AAA2707067

• Gene Names: SPOCK1; TIC1; SPOCK; TESTICAN
• Reactivity: Human
• Synonyms: Testican 1 (TIC1); Human Testican 1 (TIC1) ELISA Kit; SPOCK1; SPOCK; TESTICAN; TIC1; Sparc/Osteonectin; Swcv And Kazal-Like Domains Proteoglycan 1; TIC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Testican 1 (TIC1).; No significant cross-reactivity or interference between Testican 1 (TIC1) and analogues was observed.
• Sequence Length: 439

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Testican 1 (TIC1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Testican 1 (TIC1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIC1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Testican 1 (TIC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Testican 1 (TIC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Testican 1 (TIC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Testican 1 (TIC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4759164
• NCBI GeneID: 6695
• NCBI Accession #: NP_004589.1
• NCBI GenBank Nucleotide #: NM_004598.3
• UniProt Accession #: Q08629; Q59EW0; Q8N630; Q9UCL8; B3KSW3
• Molecular Weight: 49,124 Da
• NCBI Official Full Name: testican-1
• NCBI Official Synonym Full Names: SPARC/osteonectin, cwcv and kazal like domains proteoglycan 1
• NCBI Official Symbol: SPOCK1
• NCBI Official Synonym Symbols: TIC1; SPOCK; TESTICAN
• NCBI Protein Information: testican-1
• UniProt Protein Name: Testican-1
• UniProt Gene Name: SPOCK1
• UniProt Synonym Gene Names: SPOCK; TIC1; TICN1

NCBI Description

This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition. [provided by RefSeq, Jul 2008]

Uniprot Description

SPOCK1: May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytoplasm; extracellular space; neuromuscular junction; postsynaptic density; proteinaceous extracellular matrix; sarcoplasm

Molecular Function: calcium ion binding; cysteine-type endopeptidase inhibitor activity; metalloendopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: cell adhesion; central nervous system neuron differentiation; nervous system development; neurogenesis; neuron migration; regulation of cell growth; signal transduction