Human Cleavage And Polyadenylation Specific Factor 1 (CPSF1) ELISA Kit | CPSF1 elisa kit

Human Cleavage And Polyadenylation Specific Factor 1 (CPSF1) ELISA Kit

Cat. Num. AAA2706676

• Gene Names: CPSF1; CPSF160; P/cl.18; HSU37012
• Reactivity: Human
• Synonyms: Cleavage And Polyadenylation Specific Factor 1 (CPSF1); Human Cleavage And Polyadenylation Specific Factor 1 (CPSF1) ELISA Kit; CPSF160; Cleavage and polyadenylation specificity factor 160 kDa subunit; CPSF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cleavage And Polyadenylation Specific Factor 1 (CPSF1).; No significant cross-reactivity or interference between Cleavage And Polyadenylation Specific Factor 1 (CPSF1) and analogues was observed.
• Sequence Length: 1,443

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.129ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cleavage And Polyadenylation Specific Factor 1 (CPSF1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cleavage And Polyadenylation Specific Factor 1 (CPSF1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPSF1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cleavage And Polyadenylation Specific Factor 1 (CPSF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cleavage And Polyadenylation Specific Factor 1 (CPSF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cleavage And Polyadenylation Specific Factor 1 (CPSF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cleavage And Polyadenylation Specific Factor 1 (CPSF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119602515
• NCBI GeneID: 29894
• UniProt Accession #: Q10570; Q96AF0
• Molecular Weight: 160,884 Da
• NCBI Official Full Name: cleavage and polyadenylation specific factor 1, 160kDa, isoform CRA_b
• NCBI Official Synonym Full Names: cleavage and polyadenylation specific factor 1
• NCBI Official Symbol: CPSF1
• NCBI Official Synonym Symbols: CPSF160; P/cl.18; HSU37012
• NCBI Protein Information: cleavage and polyadenylation specificity factor subunit 1
• UniProt Protein Name: Cleavage and polyadenylation specificity factor subunit 1
• UniProt Gene Name: CPSF1
• UniProt Synonym Gene Names: CPSF160; CPSF 160 kDa subunit

NCBI Description

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied by OMIM, Mar 2008]

Uniprot Description

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.