Rat Diablo Homolog (DIABLO) ELISA Kit | DIABLO elisa kit

Rat Diablo Homolog (DIABLO) ELISA Kit

Cat. Num. AAA2706634

• Gene Names: DIABLO; SMAC; DFNA64
• Reactivity: Rat
• Synonyms: Diablo Homolog (DIABLO); Rat Diablo Homolog (DIABLO) ELISA Kit; SMAC; DIABLO-S; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; DIABLO elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Diablo Homolog (DIABLO).; No significant cross-reactivity or interference between Diablo Homolog (DIABLO) and analogues was observed.
• Sequence Length: 195

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diablo Homolog (DIABLO) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diablo Homolog (DIABLO) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DIABLO elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diablo Homolog (DIABLO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diablo Homolog (DIABLO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diablo Homolog (DIABLO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diablo Homolog (DIABLO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 507588244
• NCBI GeneID: 56616
• NCBI Accession #: NP_001265271.1
• NCBI GenBank Nucleotide #: NM_001278342.1
• UniProt Accession #: Q9NR28; Q6W3F3; Q96LV0; Q9BT11; Q9HAV6; B2RDQ0
• Molecular Weight: 22,284 Da
• NCBI Official Full Name: diablo homolog, mitochondrial isoform 3
• NCBI Official Synonym Full Names: diablo IAP-binding mitochondrial protein
• NCBI Official Symbol: DIABLO
• NCBI Official Synonym Symbols: SMAC; DFNA64
• NCBI Protein Information: diablo homolog, mitochondrial
• UniProt Protein Name: Diablo homolog, mitochondrial
• Protein Family: Diablo
• UniProt Gene Name: DIABLO
• UniProt Synonym Gene Names: SMAC; Smac

NCBI Description

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]

Uniprot Description

DIABLO: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Homodimer. Interacts with NGFRAP1/BEX3. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cytoplasmic side of plasma membrane; cytosol; mitochondrial intermembrane space; mitochondrion

Molecular Function: protein binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c; apoptosis; induction of apoptosis by oxidative stress; induction of apoptosis via death domain receptors; neuron apoptosis; positive regulation of apoptosis

Disease: Deafness, Autosomal Dominant 64