Typical Testing Data/Standard Curve (for reference only)
Human Myozenin 2 (MYOZ2) ELISA Kit | MYOZ2 elisa kit
Human Myozenin 2 (MYOZ2) ELISA Kit
Gene Names
MYOZ2; CS-1; CMH16; C4orf5
Reactivity
Human
Synonyms
Myozenin 2 (MYOZ2); Human Myozenin 2 (MYOZ2) ELISA Kit; CS-1; C4orf5; Calsarcin; Calcineurin Associated Sarcomeric Protein; FATZ-related protein 2; MYOZ2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of myostatin. No significant cross-reactivity or interference between myostatin and analogues was observed.
Sequence Length
264
Samples
Gallus Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
0.236ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level myostatin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level myostatin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MYOZ2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of myostatin in gallus serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to myostatin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to myostatin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substGalluse solution is added, only those wells that contain myostatin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substGalluse reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentGallusion of myostatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to myostatin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to myostatin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substGalluse solution is added, only those wells that contain myostatin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substGalluse reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentGallusion of myostatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
29,898 Da
NCBI Official Full Name
myozenin 2
NCBI Official Synonym Full Names
myozenin 2
NCBI Official Symbol
MYOZ2
NCBI Official Synonym Symbols
CS-1; CMH16; C4orf5
NCBI Protein Information
myozenin-2
UniProt Protein Name
Myozenin-2
Protein Family
UniProt Gene Name
MYOZ2
NCBI Description
The protein encoded by this gene belongs to a family of sarcomeric proteins that bind to calcineurin, a phosphatase involved in calcium-dependent signal transduction in diverse cell types. These family members tether calcineurin to alpha-actinin at the z-line of the sarcomere of cardiac and skeletal muscle cells, and thus they are important for calcineurin signaling. Mutations in this gene cause cardiomyopathy familial hypertrophic type 16, a hereditary heart disorder. [provided by RefSeq, Aug 2011]
Uniprot Description
Myozenins may serve as intracellular binding proteins involved in linking Z line proteins such as alpha-actinin, gamma-filamin, TCAP/telethonin, LDB3/ZASP and localizing calcineurin signaling to the sarcomere. Plays an important role in the modulation of calcineurin signaling. May play a role in myofibrillogenesis.
Research Articles on MYOZ2
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Product Notes
The Human MYOZ2 myoz2 (Catalog #AAA2706428) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2706428 ELISA Kit recognizes Human MYOZ2. It is sometimes possible for the material contained within the vial of "Myozenin 2 (MYOZ2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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