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Typical Testing Data/Standard Curve (for reference only)

Rat UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) ELISA Kit | UGT2B7 elisa kit

Rat UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) ELISA Kit

Gene Names
UGT2B7; UGT2B9; UDPGTH2; UDPGT2B7; UDPGTh-2; UDPGT 2B7; UDPGT 2B9
Reactivity
Rat
Synonyms
UDP Glucuronosyltransferase 2 Family; Polypeptide B7 (UGT2B7); Rat UDP Glucuronosyltransferase 2 Family; Polypeptide B7 (UGT2B7) ELISA Kit; UGTB2B9; UDPGTh-2; 3; 4-catechol estrogen-specific UDPGT; UGT2B7 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7).
No significant cross-reactivity or interference between UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) and analogues was observed.
Sequence Length
529
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for UGT2B7 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,695 Da
NCBI Official Full Name
UDP-glucuronosyltransferase 2B7 isoform 1
NCBI Official Synonym Full Names
UDP glucuronosyltransferase family 2 member B7
NCBI Official Symbol
UGT2B7
NCBI Official Synonym Symbols
UGT2B9; UDPGTH2; UDPGT2B7; UDPGTh-2; UDPGT 2B7; UDPGT 2B9
NCBI Protein Information
UDP-glucuronosyltransferase 2B7
UniProt Protein Name
UDP-glucuronosyltransferase 2B7
UniProt Gene Name
UGT2B7
UniProt Synonym Gene Names
UGTB2B9; UDPGT 2B7; UDPGT 2B9

NCBI Description

The protein encoded by this gene belongs to the UDP-glycosyltransferase (UGT) family. UGTs serve a major role in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This protein is localized in the microsome membrane, and has unique specificity for 3,4-catechol estrogens and estriol, suggesting that it may play an important role in regulating the level and activity of these potent estrogen metabolites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2017]

Uniprot Description

UGT2B7: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. Belongs to the UDP-glycosyltransferase family.

Protein type: Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Cofactor and Vitamin Metabolism - retinol; EC 2.4.1.17; Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - drug metabolism - other enzymes; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 4q13.2

Cellular Component: endoplasmic reticulum membrane; integral component of membrane; intracellular membrane-bound organelle; membrane

Molecular Function: glucuronosyltransferase activity; retinoic acid binding

Biological Process: androgen metabolic process; lipid metabolic process

Research Articles on UGT2B7

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Product Notes

The Rat UGT2B7 ugt2b7 (Catalog #AAA2706269) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2706269 ELISA Kit recognizes Rat UGT2B7. It is sometimes possible for the material contained within the vial of "UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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